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- Effect of petrochemical contaminants on the photosynthetic physiology on the seagrass Halophila baillonii AschersonPublication . Lima, Maria Cecilia S.; Lima, Thomaz Henrique A.; Sperandio, Marcus V. Loss; Yogui, Gilvan Takeshi; Silva, João; Magalhães, Karine M.Oil spills represent a significant threat to seagrasses as hydrocarbons can impair photosynthetic processes and plant functioning, although physiological responses may vary depending on the spill and environmental conditions This study investigates the physiological responses of Halophila baillonii when exposed to the watersoluble fraction (WSF) of crude oil. Photosynthetic pigment composition, maximum quantum yield (Fv/Fm), and oxidative stress indicators malondialdehyde (MDA) and hydrogen peroxide (H2O2) were analyzed after shortterm exposure. A significant increase in chlorophyll a and b concentrations and variations in carotenoid levels were detected, while photochemical efficiency and oxidative stress indicators were maintained. These findings suggest that the species dynamically adjusts its pigment composition to optimize light capture and mitigate oxidative stress, demonstrating physiological plasticity. Its ability to tolerate WSF exposure highlights the species' short-term resilience to hydrocarbon pollution. Future studies should investigate the effects of long-term exposure to different oil types, such as boat fuel, and the recovery mechanisms of seagrasses.
- Trait-based vulnerability and tolerance thresholds of tropical mollusks in light of projected increases in climate-change stressorsPublication . Suárez-Mozo, Nancy Yolimar; Angeles-Gonzalez, Luis Enrique; Moulatlet, Gabriel M.; Hernández-Ceballos, Ana Dianel; Díaz, Fernando; Vinagre, Catarina; Capparelli, Mariana V.Extreme climate conditions increasingly threaten worldwide coastal biodiversity. We applied a trait-based approach to quantify the tolerance thresholds of 13 mollusk species from five tropical coastal habitats, using controlled experiments that simulate extreme temperature, salinity, submersion, and desiccation stress. Survival was analyzed in relation to functional traits, including isolation from ambient conditions (presence/absence of shell closure and/or operculum), respiration mode (branchial or pulmonate), shell thickness, and habitat type. Thermal safety margins (TSMs) were also projected under future climate scenarios. Species that possess isolation traits consistently survived across stress conditions. Bivalves generally tolerated higher temperatures and broader salinity ranges than gastropods, and pulmonate gastropods experienced high mortality under both prolonged submersion and desiccation. Desiccation tolerance was strongly associated with the ability to isolate. Thicker shells provided limited protection against extreme temperatures but did not protect taxa with narrow salinity tolerances. Projected TSMs indicated that species from mangrove and oyster-bank habitats will be subject to temperatures that exceed their upper thermal limits before the end of this century. Vulnerability to climate change arises from a combination of functional traits and habitat context. By addressing multiple climate-related variables, i.e., temperature, salinity, inundation, and desiccation, all of which are shifting with global climate change, this study identified combinations of traits that will confer tolerance to tropical mollusks under future conditions and identified taxa that will likely be at risk. This study fills critical data gaps for tropical habitats worldwide and provides a framework for predicting biotic responses to climate extremes.
- Catalytic transfer hydrogenation reactions of Glycerol Over Zr-Incorporated Zeolite Beta prepared by a mechanochemical approachPublication . Fernandes, Auguste; Ribeiro, M. Filipa; Lourenço, João P.Zr-containing zeolite beta catalysts were prepared by a post-synthesis procedure involving a dealumination followed by a mechanochemical step and tested in the gas-phase conversion of glycerol. The catalytic data indicate that the catalysts are active in the conversion of glycerol, promoting not only the typical dehydration but also its hydrogenolysis without an external source of hydrogen, resulting in the production of significant amounts of ethylene glycol and methanol. Partially frameworkattached (Si─O)2Zr═O species were identified and are suggested to play an important role in the catalytic behavior of these materials.
- Families of young people who self-harm: a mixed-methods systematic review of their experiences and needsPublication . Cação-Dias, Beatriz; Rothes, Inês; Carmo, Cláudia; Nunes, Cristina; Brás, MartaBackground: Self-harm in young people is a pressing public health issue, with family support playing a crucial role in the young per son’s prognosis. Concurrently, the impact extends to families themselves, who must navigate caregiving responsibilities while also requiring support. Understanding these experiences is key to providing more effective assistance in their caregiving roles. Aim: With this mixed-methods systematic review we investigated the experiences, barriers, and needs of families of young people who self-harm. Method: Following PRISMA guidelines, a search was conducted within PsycINFO, PubMed, Web of Science, and Scopus databases in July 2024 and April 2025. Thirty-one studies were included in this review, and quality was assessed with the Mixed Methods Appraisal Tool (MMAT). A narrative synthesis was employed for the quantitative data, while qualitative data were analyzed with thematic synthesis. Results: The quantitative findings revealed two themes: (1) the repercussions of self-harm in the family and (2) family support needs. Qualitative analysis identified four themes: (1) parents’ emotional and psychological impact; (2) impact on parenting and the bond with the young person; (3) disruption of family dynamics; and (4) barriers to and pathways for family support. Conclusion: We discuss the implications of these findings, offering recommendations for future research and improvements in family support services to alleviate caregiver burden and foster supportive environments for recovery.
- Comparative proteomic analysis of trioza erytreae nymphs developed on Citrus ×limon and Citrus ×sinensis host plantsPublication . Magalhães, Tomás Thormann Abranches de; Anjos Guerreiro, Liliana Isabel Tomé; Power, Deborah Mary; Pereira, José Alberto Cardoso; Duarte, Amilcar; Tomás Marques, NatáliaTrioza erytreae is a vector of Huanglongbing (HLB), a highly damaging citrus disease. Lemon plants (Citrus ×limon) are the preferred host for T. erytreae, although the underlying mechanisms behind this remain to be fully elucidated. A comparative proteomic analysis of T. erytreae nymphs in their fourth and fifth instars that were fed either lemon or sweet orange (SwO) was carried out to investigate the interaction with its hosts. A 24-hour sucrose feeding assay was conducted to understand proteomic responses to a nutrient-poor diet. Proteomic profiling using nanoscale liquid chromatography coupled to tandem mass spectrometry (nanoLC-MS/MS) identified a total of 1,477 psyllid proteins with high confidence. Oviposition and nymphal development were also evaluated across citrus hosts, revealing higher numbers of nymphs developing on lemon than on SwO. Feeding on SwO enriched pathways related to “transmission across chemical synapses” and “metabolism of proteins”. Responses observed under a 24-hour sucrose-only diet enriched the biological processes “response to external stimulus”, “response to stress” and “cytoskeleton organization”. In contrast, these enrichments were absent on lemon host, suggesting that lemon provides a more favourable environment for psyllid development. In addition, nymphs developed on lemon exhibited enhanced energy metabolism and an increase in translation initiation factors. Overall, the results demonstrate that development strongly depends on host plant species, with SwO impairing optimal growth and lemon promoting successful nymphal development.
- The effects of country governance quality on corporate sustainability and ethical behaviourPublication . Francisco, Paulo MoraisUsing institutional theory, we examine how country governance affects two ESG outcomes: ESG performance and ESG controversies. With Refinitiv/LSEG data for ~146,000 firm‐years in 86 countries (2002–2023) and World Bank WGI, we apply a Mundlak within/between decomposition to test complementarity versus substitution (performance) and prevention versus detection (controversies). Better governance is strongly associated with higher ESG performance—overall and across E, S and G—especially cross‐country. Yet governance also predicts more reported controversies, consistent with detection/visibility rather than worse conduct. Instrumental Variable and DiD tests corroborate these results. JEL Classification: D02, M14, Q56, C23
- Bioprospecting fungi in mediterranean fermentations: functional insights and antibacterial potentialPublication . Bertan, David Willian; Kamimura, Eliana Setsuko; Quintas, CéliaTable olives, particularly traditionally fermented cracked-style green olives, rely on natural microbial activity without chemical debittering, with fungi playing key roles; in contrast, arbutus berry fermentation remains less characterized in terms of microbial functionality. This study investigated the enzymatic and antibacterial potential of fungal isolates from both systems. A total of 84 isolates belonging to Aureobasidium, Candida, Cryptococcus, Saccharomyces, Pichia, Issatchenkia, Torulaspora, and Sporobolomyces were screened for hydrolytic enzymes (pectinases, amylases, cellulases, xylanases, lipases, proteases, tannases, and β-glucosidases) using selective media, and for antibacterial activity against major foodborne pathogens. Isolates from arbutus fermentation showed no relevant enzymatic or antibacterial ability. In contrast, several isolates from olive fermentation exhibited significant functional traits. Aureobasidium pullulans demonstrated broad enzymatic capacity, producing amylases, esterases, and tannases, along with lipid hydrolysis, but also expressed cellulase, pectinase, and protease abilities. Cryptococcus spp. displayed interesting profiles, with low cellulolytic and pectinolytic capacity and higher phenolase, esterase, and lipase capacities. Antibacterial activity was observed exclusively against Gram-positive bacteria, particularly Staphylococcus aureus and Listeria monocytogenes, mainly among Candida membranifaciens, Cryptococcus spp., and A. pullulans. Overall, table olive fermentation isolates showed promising biotechnological potential for food preservation and quality enhancement, whereas arbutus isolates appeared to have limited functional relevance.
- Holisticscape dimensions of the tourism experience in wellness spas: Evidence from PortugalPublication . Valente Pedro, Cristina; de Matos, Nelson Manuel da Silva; Pinto, PatríciaThe present study examines how tourists’ experiences in wellness spas promote holistic health through the Holisticscape framework from both tourists’ and other stakeholders’ perspectives, an extension of the servicescape that encompasses the ambience, social, activity, body, mind, and spirit dimensions. An ex-ploratory, multi-stakeholder qualitative design was employed, with 20 in-depth interviews conducted inwellness spas in Portugal. Thematic analysis revealed that ambience, social relations, activity, body, mind, and spiritual stimuli support tourists’ holistic health. These findings extend existing research on servicescape and experiencescape by demonstrating how multidimensional experiential stimuli interact to produce holistic health in wellness spa settings. The study offers practical implications for designing immersive and health-oriented environments, enhancing service customisation, and integrating cultural and natural resources intowellness spa offerings. Given its exploratory and context-bound nature, it is recommended that further research be conducted to validate Holisticscape dimensions across a range of wellness spas at distinct destinations. Furthermore, the development of measurement tools for future quantitative investigations is recommended.