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- A model for signal transduction during gamete release in the fucoid alga Pelvetia compressaPublication . Pearson, Gareth; Brawley, S. H.Fucoid algae release gametes into-seawater following an inductive light period (potentiation), and gamete expulsion from potentiated receptacles of Pelvetia compressa began about 2 min after a light-to-dark transition. Agitation of the medium reversed potentiation, with an exponential time course completed in about 3 h. Light regulated two signaling pathways during potentiation and gamete expulsion: a photosynthetic pathway and a photosynthesis-independent pathway in which red light was active but blue light was not. Uptake of K+ appears to have an important role in potentiation, because a 50% inhibition of potentiation occurred in the presence of the tetraethylammonium ion, a K+-channel blocker. A central role of anion channels in the maintenance of potentiation is suggested by the premature release of gametes in the light when receptacles were incubated with inhibitors of slow-type anion channels. An inhibitor of tyrosine kinases, tyrphostin A63, also inhibited potentiation. A model for gamete release from P. compressa is presented that proposes that illumination results in the accumulation of ions (e.g. K+) throughout the cells of the receptacle during potentiation, which then move into the extracellular matrix during gamete expulsion to generate osmomechanical force, resulting in gamete release.
- Control of gamete release in fucoid algae: sensing hydrodynamic conditions via carbon acquisitionPublication . Pearson, G. A.; Serrão, Ester; Brawley, S. H.We investigated the environmental factors providing signals for gamete release in fucoid algae, with a particular focus on the inhibitory effect of water motion. The release of gametes by Fucus distichus occurred in still water under high light and was associated with the depletion of dissolved inorganic carbon (DIC) in tide pools isolated from the ocean during daytime low tides. Diurnal patterns of gamete release that lasted for 2-3 d, as in natural populations, were found in receptacles cultured in a 12 h light: 12 h dark photoperiod under calm conditions or in a simulated tidal regime. Constant light disrupted the diurnal component of release, however, suggesting that an endogenous circadian periodicity was absent. The effects of increased [DIC] on cultured receptacles under calm conditions were similar to the inhibitory effects of high water motion; both conditions reduced release in several species from both tide pool and intertidal habitats. Bicarbonate concentration, rather than carbon dioxide, or carbonate, was shown to be the component of the DIC system most closely correlated (inversely) with gamete release in the intertidal fucoid Pelvetia compressa. The hypothesis that gamete release is triggered by DIC depletion in the unstirred boundary layer during periods of low water motion in the light was further supported by experiments with P. compressa. These showed that gamete release was not inhibited by high water motion when DIC was absent. In the same experiments, high water motion inhibited release at 2 and 20 mmol/L DIC, confirming that the sensitivity of gamete release to water motion is DIC dependent. The ability of fucoid algae to couple physiological processes (e.g., photosynthesis) with life history events (gamete release) allows external fertilization to occur under favorable hydrodynamic conditions, and may contribute greatly to reproductive success in organisms inhabiting periodically turbulent environments.
- Successful external fertilization in turbulent environmentsPublication . Serrão, Ester; Pearson, G. A.; Kautsky, L.; Brawley, S. H.Mathematical and experimental simulations predict that external fertilization is unsuccessful in habitats characterized by high water motion. A key assumption of such predictions is that gametes are released in hydrodynamic regimes that quickly dilute gametes. We used fucoid seaweeds to examine whether marine organisms in intertidal and subtidal habitats might achieve high levels of fertilization by restricting their release of gametes to calm intervals. Fucus vesiculosus L. (Baltic Sea) released high numbers of gametes only when maximal water velocities were below ca. 0.2 m/s immediately prior to natural periods of release, which occur in early evening in association with lunar cues. Natural fertilization success measured at two sites was always close to 100%. Laboratory experiments confirmed that (i) high water motion inhibits gamete release by F. vesiculosus and by the intertidal fucoids Fucus distichus L. (Maine) and Pelvetia fastigiata (J. Ag.) DeToni (California), and (ii) showed that photosynthesis is required for high gamete release. These data suggest that chemical changes in the boundary layer surrounding adults during photosynthesis and/or mechanosensitive channels may modulate gamete release in response to changing hydrodynamic conditions. Therefore, sensitivity to environmental factors can lead to successful external fertilization, even for species living in turbulent habitats.
- Gamete release at low tide in fucoid algae: Maladaptive or advantageous?Publication . Brawley, S. H.; Johnson, L. E.; Pearson, G. A.; Speransky, V.; Li, R.; Serrão, EsterThis review discusses three questions pertaining to gamete release by fucoid algae at low tide: 1) Are gametes viable and does fertilization occur at low tide?, 2) How many gametes are released at low tide versus at high tide? and 3) Is gamete release at low tide maladaptive or is it selectively advantageous? Gamete release at low tide (LT) in fucoid algae is observed commonly in monoecious species from the lower and mid-intertidal zones (e.g., Fucus distichus, F. evanescens, F. gardneri, Pelvetia compressa); in dioecious species, intact antheridia (containing sperm) are commonly released at low tide (e.g., in Ascophyllum nodosum, F. vesiculosus). Fertilization at low tide can be determined with a calcofluor white assay and occurs in at least three species (F. distichus, F. gardneri, P. compressa). In general, fucoid algae have high levels of fertilization success, but substantial mortality occurs during early embryogenesis in some intertidal zones due to physical stresses. The agarose bead assay is useful to assess desiccation on an egg-sized scale. Constitutive dehydrin-like proteins are present in sperm, eggs, and embryos. The release of gametes at low tide appears to be a consequence of a mechanism selected to permit gamete release under calm conditions in seawater; this mechanism is described. The timing of adhesion by zygotes at low and high tide is unknown. Such information and additional data on the relative proportions of zygotes produced by monecious species at low tide versus high tide are required in order to assess effects of gamete release at low tide upon dispersal and population structure.