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- Cloning and sequencing of a full-length sea bream (Sparus aurata) beta-actin cDNAPublication . Santos, Cecilia; Power, Deborah; Kille, Peter; Llewellyn, Lynda; Ramsurn, Vimi P.; Wigham, Trevor; Sweeney, Glen E.A full-length cDNA clone encoding beta-actin (b-actin) was isolated from a sea bream (Sparus aurata) liver cDNA library. Sequencing of this clone reveals an open reading frame encoding a 375 amino acid protein that shares a high degree of conservation to other known actins. The sea breamb-actin sequence showed 98% identity to carp and human b-actin and 95% and 94% identity to sea squirt and Dictyostelium cytoplasmic actins, respectively.