Cloning and sequencing of a full-length sea bream (Sparus aurata) beta-actin cDNA

Santos, Cecilia; Power, Deborah; Kille, Peter; Llewellyn, Lynda; Ramsurn, Vimi P.; Wigham, Trevor; Sweeney, Glen E.

http://hdl.handle.net/10400.1/5450

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Resumo(s)

A full-length cDNA clone encoding beta-actin (b-actin) was isolated from a sea bream (Sparus aurata) liver cDNA library. Sequencing of this clone reveals an open reading frame encoding a 375 amino acid protein that shares a high degree of conservation to other known actins. The sea breamb-actin sequence showed 98% identity to carp and human b-actin and 95% and 94% identity to sea squirt and Dictyostelium cytoplasmic actins, respectively.

Palavras-chave

b-actin cDNA Conservation Evolution Sea bream (Sparus aurata) Teleost

URI

http://hdl.handle.net/10400.1/5450

Citação

Cecilia R. A. Santos, Deborah M. Power, Peter Kille, Lynda Llewellyn, Vimi Ramsurn, Trevor Wigham, and Glen E. Sweeney. Cloning and Sequencing of a Full-Length Sea Bream (Sparus aurata) b-Actin cDNA. Comp. Biochem. Physiol. Vol. 117B, No. 2, pp. 185–189, 1997.

Editora

Elsevier

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