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- Complementary sampling methods to improve the monitoring of Coastal LagoonsPublication . Adão, Ana Catarina; Bosch, Néstor E.; Bentes, Luis; Coelho, Rui; Lino, Pedro G.; Monteiro, Pedro; Gonçalves, Jorge Manuel Santos; Erzini, KarimMonitoring the ecological status of marine coastal lagoons requires the integration of multiple indices. However, the efficacy of monitoring programs is complicated by the diverse array of habitats that conform coastal lagoons. In this study, we compared four sampling methods (25-m and 50-m beach seines, beam trawl and Riley push net) in the Ria Formosa coastal lagoon (South Portugal) for assessing fish assemblage and diversity. We compared species richness and assemblage structure with species accumulation curves and multivariate analysis, and assessed diversity patterns using taxonomic, phylogenetic and functional diversity indices. Variation in fish assemblage structure was mostly explained by gear type, and almost all diversity metrics varied not only according to sampling method but also depending on habitat characteristics and season. Based on operational costs and diversity patterns captured by each gear, we conclude that the combined use of 25-m beach seine and beam trawl is the preferred approach. The proposed methodology will provide the data necessary for assessment of ecological status of coastal lagoons.