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- Single-Strand Conformation Polymorphism (SSCP) analysis of alfas1-casein, beta-casein and k-casein genes in Charnequeira Portuguese indigenous goat breedPublication . Bonifácio, C.; Santos, Ingrid; Belo, Carmona; Cravador, A.The DNA from eighty goats belonging to the indigenous portuguese caprine breed Charnequeira, ecotype from Beira Baixa (Beiroa), was analysed. Single-strand conformation polymorphisms were identified at exon 9 and at exons 10-11 of the as1-casein gene and at exon 4 of the kcasein. The b-casein gene was found monomorphic at exon 7 in this population sample. The establishment of an association of some of these SSCP polymorphisms with milk production and protein and fat content was attempted.