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- Exploring the halophyte Cistanche phelypaea (L.) Cout as a source of health promoting products: In vitro antioxidant and enzyme inhibitory properties, metabolomic profile and computational studiesPublication . Trampetti, Francesca; Pereira, Catarina; Rodrigues, Maria Joao; Celaj, Odeta; D'Abrosca, Brigida; Zengin, Gokhan; Mollica, Adriano; Stefanucci, Azzurra; Custódio, LuísaIn this study, ethyl acetate, acetone, ethanol and water extracts from flowers, stems and roots of Cistanche phelypaea (L.) Cout were appraised for radical scavenging activity (RSA) towards 1,1-diphenyl-2-picrylhydrazyl,2,2-azino-bis(3-ethylbenzo-thiazoline-6-sulfonic acid) and superoxide free radicals, and for metal chelating activities on iron and copper ions. The water extracts had the highest antioxidant activity, especially those from roots and flowers, and were further appraised for in vitro inhibition of enzymes implicated on the onset of human ailments, namely acetyl- (AChE) and butyrylcholinesterase (BuChE) for Alzheimer's disease, alpha-glucosidase and alpha-amylase for diabetes, and tyrosinase for skin hyper-pigmentation disorders. The extracts had a higher activity towards BuChE, and the roots extract had the highest capacity to inhibit tyrosinase. Samples showed a low capacity to inhibit carbohydrate hydrolysing enzymes, except for the root extract with a good inhibition on glucosidase. Samples were then characterized by NMR (1D and 2D): the main metabolites identified in the flowers extract were iridoid glycosides, in particular gluroside and bartsioside. In stems, phenylehanoid glycosides (PhGs) and iri doids were detected, especially acteoside. In roots were detected essentially PhGs, mainly echinacoside and tubuloside A. Docking studies were performed on the identified compounds. A favorable binding energy of tubuloside A to tyrosinase was calculated, and indicated this compound as a possible competitive inhibitor of alpha-glucosidase and tyrosinase. Our results suggest that C. phelypeae is a promising source of biologically-active compounds with health promoting properties for pharmaceutical and biomedical applications. (C) 2018 Elsevier B.V. All rights reserved.
- Natural products from extreme marine environments: searching for potential industrial uses within extremophile plantsPublication . Lopes, Andreia; Rodrigues, Maria João; Pereira, Catarina; Oliveira, Marta; Barreira, Luísa; Varela, J.; Trampetti, Francesca; Custódio, LuísaExtremophile plants, halophytes in particular, still remain largely unexplored and underutilized despite their high potential as source of industrial raw materials like natural antioxidants in industries related to pharmaceutical, cosmetics and functional ingredients. The goal of this study was to explore the phenolic content and the in vitro antioxidant and tyrosine inhibition properties of polyphenol-rich extracts of twenty-one extremophile plant species collected in saline habitats of the South of Portugal (Algarve). Aqueous acetone extracts of Lithrum salicaria, Cladium mariscus, Frankenia laevis, F. pulverulenta, Limoniastrum monopetalum, Aster tripolium and Typha domigensis had remarkable levels of total phenolics (>= 230 mg GAE/g DW); Halopeplis amplexicaulis, Cladium mariscus,F. puiverulenta and Salsola vermiculata contained high levels of flavonoids (>= 10 mg RE/g DW); tannins peaked in T. domingensis and C. mariscus (>= 35 mg CE/g DW) and L. salicaria and A. tripolium had the upmost hydrocinamic acids content (>= 60 mg CAE/g DW). Overall L. salicaria had the highest antioxidant activity and only P. lentiscus had the capacity to inhibit tyrosinase. As the most bioactive extracts were not cytotoxic towards mammalian cell lines, our results suggest that several halophytes present in Southern Portugal could be used in the food and/or cosmetic industries.