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- SPIN enables high throughput species identification of archaeological bone by proteomicsPublication . Rüther, Patrick Leopold; Husic, Immanuel Mirnes; Bangsgaard, Pernille; Gregersen, Kristian Murphy; Pantmann, Pernille; Carvalho, Milena; Godinho, Ricardo Miguel; Friedl, Lukas; Cascalheira, João; Taurozzi, Alberto John; Jørkov, Marie Louise Schjellerup; Benedetti, Michael; Haws, Jonathan; Bicho, Nuno; Welker, Frido; Cappellini, Enrico; Olsen, Jesper VelgaardSpecies determination based on genetic evidence is an indispensable tool in archaeology, forensics, ecology, and food authentication. Most available analytical approaches involve compromises with regard to the number of detectable species, high cost due to low throughput, or a labor-intensive manual process. Here, we introduce “Species by Proteome INvestigation” (SPIN), a shotgun proteomics workflow for analyzing archaeological bone capable of querying over 150 mammalian species by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Rapid peptide chromatography and data-independent acquisition (DIA) with throughput of 200 samples per day reduce expensive MS time, whereas streamlined sample preparation and automated data interpretation save labor costs. We confirm the successful classification of known reference bones, including domestic species and great apes, beyond the taxonomic resolution of the conventional peptide mass fingerprinting (PMF)-based Zooarchaeology by Mass Spectrometry (ZooMS) method. In a blinded study of degraded Iron-Age material from Scandinavia, SPIN produces reproducible results between replicates, which are consistent with morphological analysis. Finally, we demonstrate the high throughput capabilities of the method in a high-degradation context by analyzing more than two hundred Middle and Upper Palaeolithic bones from Southern European sites with late Neanderthal occupation. While this initial study is focused on modern and archaeological mammalian bone, SPIN will be open and expandable to other biological tissues and taxa.
- Mapping the stone age of MozambiquePublication . Gonçalves, Célia; Raja, Mussa; Madime, Omar; Cascalheira, João; Haws, Jonathan; Matos, Daniela; Bicho, Nuno Gonçalo Viana Pereira FerreiraUnder the auspices of the Portuguese colonial government, Lereno Barradas and Santos Junior (coordinator of the Anthropological Mission of Mozambique) carried out several archaeological field surveys from 1936 to 1956 that resulted in a data set that includes a total of close to 90 sites, mostly attributed to the Stone Age. This early research added to the previous work of Van Riet Lowe in the Limpopo Valley of southern Mozambique. With the new millennium, Mozambique has emerged as a crucial geographic area in which to understand the various hypotheses about recent human evolution. Specifically, its coastal location between southern and eastern Africa is ideal for testing ideas about the link between early coastal adaptations and the appearance of anatomically modern humans (AMH). Except for the recent work by Mercader's team in northern Mozambique, the number of researchers and projects on this topic in Mozambique is still limited because of the general predominance of interest in later periods among archaeologists working in the country, mainly due to their focus on issues related to precolonial heritage and national identity. Based on the early maps from Santos Junior and more recent data acquired through various projects, we present a series of maps for the Stone Age prehistory of Mozambique. The maps are also based on a critical evaluation of the sites and a review of some of the materials that are presently curated at the Instituto de Investigacao Cientifica e Tropical (IICT) in Lisbon, Portugal, as well as the materials stored at the Department of Archaeology of Universidade Eduardo Mondlane, Maputo. The sites are also presented in an online database with the information on all sites used in this study. This database is open to all and will be updated continuously. A preliminary interpretation of the regional distribution of the sites is also attempted, linking aspects that include region, topography and altitude, geomorphology, and cultural phase. These results will be the first step for research and knowledge in Mozambique on Stone Age prehistory and the emergence and settlement pattern of AMH.
- The Gravettian-Solutrean transition in westernmost Iberia: new data from the sites of Vale Boi and Lapa do PicareiroPublication . Belmiro, Joana; Bicho, Nuno; Haws, Jonathan; Cascalheira, JoãoThis study presents the analysis of the lithic assemblages from Layers 5 and 4E (Terrace) of the site of Vale Boi (southern Portugal) and Levels U and T from Lapa do Picareiro (central Portugal). We aimed to understand the technological patterns and raw material exploitation during the Gravettian-Solutrean transition in westernmost Iberia and test the traditional models with assemblages from recently excavated sites, while expanding the geographic range. Results show the existence of two discrete phases in each site. The first, with high frequency of quartz use for bladelet production, seems to reflect the presence of a Terminal Gravettian horizon, as defined by Almeida (2000). The second, with some significant differences between sites, attests the presence of Vale Comprido technology and lower quartz frequencies at Vale Boi, representing a Proto-Solutrean occupation; and the presence of a blade component in Lapa do Picareiro that, together with the respective absolute chronology, may be attributed to a Proto-Solutrean or an Early Solutrean horizon. In general terms, this study allowed to confirm that the Terminal Gravettian and the Proto-Solutrean are discrete phases across the transition, in agreement with the Three-Phase model presented by Zilh˜ao (1997). It further consolidates the expansion of similar techno-cultural patterns to southern Portugal, which may be explained by the significant changes in the dynamic of social networks (Cascalheira and Bicho, 2013).
- The early Aurignacian dispersal of modern humans into westernmost EurasiaPublication . Haws, Jonathan; Benedetti, Lucilla; Talamo, Sahra; Bicho, Nuno Gonçalo Viana Pereira Ferreira; Cascalheira, João; Ellis, M. Grace; Carvalho, Milena M.; Friedl, Lukas; Pereira, Telmo; Zinsious, Brandon K.Documenting the first appearance of modern humans in a given region is key to understanding the dispersal process and the replacement or assimilation of indigenous human populations such as the Neanderthals. The Iberian Peninsula was the last refuge of Neanderthal populations as modern humans advanced across Eurasia. Here we present evidence of an early Aurignacian occupation at Lapa do Picareiro in central Portugal. Diagnostic artifacts were found in a sealed stratigraphic layer dated 41.1 to 38.1 ka cal BP, documenting a modern human presence on the western margin of Iberia ∼5,000 years earlier than previously known. The data indicate a rapid modern human dispersal across southern Europe, reaching the westernmost edge where Neanderthals were thought to persist. The results support the notion of a mosaic process of modern human dispersal and replacement of indigenous Neanderthal populations.
- Human adaptive responses to climate and environmental change during the Gravettian of Lapa do Picareiro (Portugal)Publication . Haws, Jonathan; Benedetti, Michael; Carvalho, Milena; Ellis, Grace; Pereira, Telmo; Cascalheira, João; Bicho, Nuno; Friedl, LukasOn the Iberian Peninsula, abrupt climate shifts during the Late Pleistocene impacted human and natural systems. Our knowledge of human adaptive responses to these climatic perturbations has improved in recent years with the development of new radiocarbon techniques that have increased the temporal resolution of cultural chronologies. At the same time, new high-resolution paleoclimatic records from Greenland ice cores, deep-sea sediment cores, speleothems, and microfaunal assemblages have permitted detailed paleoenvironmental reconstructions. Combined with the archaeological record of culture change, these data sets allow for a better understanding of the nature of human socio-ecological systems during the Late Pleistocene. Here we present new data on the Gravettian occupations at Lapa do Picareiro, a cave site in Portugal with a long continuous stratigraphic sequence spanning MIS 3 and 2. The ongoing excavation of Lapa do Picareiro has revealed at least three Gravettian levels with very different patterns of raw material use and technological reduction. The Early Gravettian levels contain a quartzite flake assemblage with several refitting clusters and a small chert assemblage with nosed endscrapers and bladelet technology. The Late or Terminal Gravettian level contains carinated endscraper/core-bladelet technology, mainly quartz, with chert blade production, and a few bone tools. The faunal assemblages also have a different character. Both contain a similar range of medium and large ungulates but the earlier Gravettian has a much richer and more diverse assemblage of small animals. These combined archaeological data sets provide a new perspective on Gravettian human adaptations in response to climate shifts, especially Heinrich Event 3 and Greenland Interstadials 4 and 3.
- Middle and late stone age of the Niassa region, Northern Mozambique. Preliminary resultsPublication . Bicho, Nuno Gonçalo Viana Pereira Ferreira; Haws, Jonathan; Raja, Mussa; Madime, Omar; Gonçalves, Célia; Cascalheira, João; Benedetti, Michael M.; Pereira, Telmo; Aldeias, VeraLocated between modern-day South Africa and Tanzania, both of which have well-known and extensive Stone Age records, Mozambique's Stone Age sequence remains largely unknown in the broader context of African Pleistocene prehistory. Such lack of data occurs despite the key geographical location of the country, in southern Africa at the southeastern tip of the Great Rift Valley. As such, Mozambique is an area of interest to evaluate the origins and dispersion of Homo sapiens within Africa, particularly in relation to Middle Stone Age contexts and associated early modern human ecology and cognition.This paper focuses on preliminary survey results from the Niassa District, near Lake Niassa (also known as Lake Malawi) in northern Mozambique. The results include the discovery and location of more than 80 new surface lithic concentration localities, as well as data from two new sites, the open air surface site of Ncuala and the rock shelter of Chicaza. For Chicaza we provide a series of new radiocarbon dates for the Iron Age and Late Stone Age occupations based on preliminary testing carried out at the site. (C) 2015 Elsevier Ltd and INQUA. All rights reserved.
- Portable art and personal ornaments from Txina-Txina: a new later stone age site in the Limpopo River Valley, southern MozambiquePublication . Bicho, Nuno Gonçalo Viana Pereira Ferreira; Cascalheira, João; André, Lino; Haws, Jonathan; Gomes, Ana; Gonçalves, Célia; Raja, Mussa; Benedetti, Michael M.This paper reports on preliminary fieldwork at the Later Stone Age site of Txina-Txina in Mozambique. Excavation yielded a long stratigraphic sequence, a large lithic assemblage, a unique decorated gastropod shell fragment and two ostrich eggshell beadsthe first of their type recovered from a Stone Age context in Mozambique.