A carregar...
3 resultados
Resultados da pesquisa
A mostrar 1 - 3 de 3
- Insights from bacteroides species in children with Type 1 DiabetesPublication . Matos, José; Matos, Isabel; Calha, Maria; Santos, Pedro; Duarte, Isabel; Cardoso, Yameric; Faleiro, Maria Leonor: In our previous study the enrichment of the intestinal proteome of type 1 diabetes (T1D) children with Bacteroides proteins was observed, which led us to our current study that aimed to isolate and characterize Bacteroides species from fecal samples of T1D and control children. Repetitive sequence-based PCR (rep-PCR) was used for typing the isolated Bacteroides species. The antibiotic susceptibility and mucinolytic activity of the isolates was determined. The quantification of specific bacterial groups in the fecal samples was determined by qPCR. The ability to adhere and invade the human colonic cell line HT29-MTX-E12 of strains of P. dorei, B. uniformis and P. distasonis was determined and their whole genome sequencing was performed. The results showed similar numbers of Bacteroides species in T1D and control samples, but unique Bacteroides species and a higher recovery of P. distasonis from T1D samples was observed. Rep-PCR grouped the different Bacteroides species, but no discrimination by origin was achieved. T1D children showed a significant increase in Proteobacteria and a depletion in Lactobacillus sp. All tested P. dorei, B. uniformis and P. distasonis were able to adhere to HT29-MTX-E12 cells but significant differences (p < 0.05) in the ability to invade was observed. The highest ability to invade was exhibited by P. distasonis PtF D14MH1 and P. dorei PtFD16P1, while B. uniformis strains were unable to invade. The damage to tight junctions was also observed. The presence of Lactobacillus sp. inhibited the invasion ability of P. distasonis PtF D14MH1 but not P. dorei PtFD16P1. Sequences of agonist peptides of the human natural preproinsulin and the insulin B chain insB:9-23 peptide mimics were identified. The results reported in our study stresses the continued efforts required to clarify the link between T1D and gut microbiota.
- Chemical characterization and biological properties assessment of Euphorbia resinifera and Euphorbia officinarum Moroccan PropolisPublication . Boutoub, Oumaima; El-Guendouz, Soukaina; Matos, Isabel; El Ghadraoui, Lahsen; Costa, Maria Clara; Carlier, Jorge; Faleiro, Maria Leonor; Figueiredo, Ana Cristina; Estevinho, Letícia M.; Miguel, MariaAlthough the plants of the genus Euphorbia are largely exploited by therapists in Morocco, the composition and antibacterial activities of propolis from these plants are still unknown. To address this gap, this study aimed to characterize the pollen type, the volatile compounds, and the phenolic and mineral profiles of three Euphorbia propolis samples collected in Morocco and evaluate their antimicrobial activities. The minimum inhibitory concentration of the propolis samples was determined by the microdilution method, and the anti-adherence activity was evaluated by the crystal violet assay. The examination of anti-quorum-sensing proprieties was performed using the biosensor Chromobacterium violaceum CV026. Pollen analysis revealed that Euphorbia resinifera pollen dominated in the P1 sample (58%), while E. officinarum pollen dominated in the P2 and P3 samples (44%). The volatile compounds were primarily composed of monoterpene hydrocarbons, constituting 35% in P1 and 31% in P2, with α-pinene being the major component in both cases, at 16% in P1 and 15% in P2. Calcium (Ca) was the predominant mineral element in both E. resinifera (P1) and E. officinarum (P2 and P3) propolis samples. Higher levels of phenols, flavonoids and dihydroflavonoids were detected in the E. officinarum P2 sample. The minimum inhibitory concentration (MIC) value ranged from 50 to 450 µL/mL against Gram-positive and Gram-negative bacteria. Euphorbia propolis displayed the ability to inhibit quorum sensing in the biosensor C. violaceum CV026 and disrupted bacterial biofilm formation, including that of resistant bacterial pathogens. In summary, the current study evidences the potential use of E. officinarum propolis (P2 and P3) to combat important features of resistant pathogenic bacteria, such as quorum sensing and biofilm formation.
- The conversion of rockrose (Cistus ladanifer L.) shrubland into biodiverse pasturesPublication . Vieira, João; Filip, Iván Daniel; Soares Curado de Matos, Isabel Cristina; Rodrigues, Alessandra; Trindade, Ana Rita; Silva, Luís; Cassinello, João; Carita, Teresa; Ferreira da Silva da Costa Freitas, Maria de Belém; Neves, Maria AlcindaPastures in the Mediterranean region are vital for sustaining local agriculture and preserving biodiversity. When well-managed, these pastures enhance soil structure and fertility, reduce erosion, and improve water retention. These functions are especially critical in a climate marked by hot, dry summers and mild, wet winters. Moreover, these systems play a key role in carbon sequestration, contributing significantly to climate change mitigation. Mediterranean pastures also support the conservation of endemic plant species and provide essential habitats for a variety of wildlife. Quinta do Freixo (Algarve, Portugal) with an area around 800 hectares, is dedicated to sustainable agriculture and promotes ecological, economic and social sustainability. In addition to organic agriculture production, the farm also produces value-added food products and offers rural tourism experiences. On this farm, a dense shrubland of rockrose (Cistus ladanifer L.) is converted into pastures using a holistic method that includes mechanical cutting of the shrubs, spreading hay an regenerative rotational grazing (RRG) system. As a part of the project -C05-i03-I000237), these restored pastures are regularly monitored. Assessments included measurements of dry matter production and floristic composition. In addition, both the pastures and Cistus ladanifer shrublands were evaluated for the nutritional value and mineral composition of their foliage material. This approach has contributed to the development of species-rich pastures, rich in legumes and grasses and characterized by high protein content, excellent digestibility, and a well-balanced mineral composition. Although Cistus ladanifer had a relatively lower nutritional value due to its lower protein content and digestibility, the analysis showed that its aerial parts can serve as a supplementary feed resource, especially in periods of forage scarcity, contributing to overall feed availability in extensive grazing systems.The control of shrublands through this practice maintains biodiversity and reduces the risk of wildfires by effectively managing fuel loads.