CRIOPRESERVAÇÃO DE SÉMEN E EMBRIÕES DE PEIXES TELEÓTEOS

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Contribuição parental do linguado do Senegal, Solea senegalensis (KAUP 1858), em aquacultura

Publication . Madeira, Céline Rodrigues; Cancela, Leonor; Mira, S.

Since the early 80?s Senegalese sole (Solea senegalensis, Kaup 1858) has been a potential target for intensive cultivation in Southern Europe, due to its high economic value. However, there are still some limitations in particular those related to the existing difficulties in controlling reproduction in captivity. In aquaculture, managing the number of required breeding individuals is important to produce viable and variable offspring to ensure its survival, avoiding high levels of consanguinity and its deleterious effects. From an economic standpoint it is also essential to ensure that breeding individuals contribute efficiently to a viable offspring. In this study, the contribution of each of 10 adult individuals to the offspring was analysed by testing approximately 25 eggs per posture daily. Samples of postures were daily collected between March and May 2007. After DNA extraction, the paternity of individual eggs was determined using 7 microsatellites loci (SSeGATA38, SolMII, SolCA13, Sol13D, Sol5D, SolGA12, SseCA39). The set of 7 microsatellites loci analyzed has a high probability of exclusion thus making them indispensable tools in the allocation of paternidades. However, the high percentage of genotyping errors due to the presence of null alleles can be a problem. This study demonstrated a clear dominance from a couple of progenitors throughout the entire reproduction season. The results obtained showed the usefulness of this methodology to characterise the groups of effective breeders in this species.

2008Master thesis

Open Access