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Authors
Advisor(s)
Abstract(s)
Since the early 80?s Senegalese sole (Solea senegalensis, Kaup 1858) has been a
potential target for intensive cultivation in Southern Europe, due to its high economic
value. However, there are still some limitations in particular those related to the existing
difficulties in controlling reproduction in captivity. In aquaculture, managing the
number of required breeding individuals is important to produce viable and variable
offspring to ensure its survival, avoiding high levels of consanguinity and its deleterious
effects. From an economic standpoint it is also essential to ensure that breeding
individuals contribute efficiently to a viable offspring.
In this study, the contribution of each of 10 adult individuals to the offspring was
analysed by testing approximately 25 eggs per posture daily. Samples of postures were
daily collected between March and May 2007. After DNA extraction, the paternity of
individual eggs was determined using 7 microsatellites loci (SSeGATA38, SolMII,
SolCA13, Sol13D, Sol5D, SolGA12, SseCA39). The set of 7 microsatellites loci
analyzed has a high probability of exclusion thus making them indispensable tools in
the allocation of paternidades. However, the high percentage of genotyping errors due to
the presence of null alleles can be a problem. This study demonstrated a clear
dominance from a couple of progenitors throughout the entire reproduction season. The
results obtained showed the usefulness of this methodology to characterise the groups of
effective breeders in this species.
Description
Dissertação mest., Biologia Marinha, Universidade do Algarve, 2008
Keywords
Teses Aquacultura Linguado