MAPEAMENTO GENÉTICO DE ANANÁS

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Mapeamento genético do ananaseiro (Ananas comosus (L.) Merrilll)

Publication . Carlier, Jorge Daniel Dias; Leitão, José Manuel Peixoto Teixeira

The main aim of this thesis was the construction of a Pineapple (Ananas comosus (L.) Merrill) genetic reference map. Based on an (A. comosus var. comosus X Ananas comosus var. bracteatus) F1 population, two genetic maps, one for each progenitor, were assembled. Subsequently, a new map integrating markers from both progenitors was constructed on the basis of an F2 population. Using markers segregating simultaneously among the F1 and F2 populations, an integrated map was constructed gathering 783 molecular markers (588 AFLP, 137 RAPD, 57 ISSR, and the PGM isozyme locus) and the morphological trait locus “piping” into 77 linkage groups. In view of the facts that (1) in the integrated map, 31 linkage groups (a number close to the 25 pineapple chromosomes) are longer than 50cM; (2) that the different maps span over 60.7% to 91.4% of the genome, and; (3) that marker density is moderately high, with 4.16 cM average distance between markers, it is legitimate to suppose that these first genetic maps of pineapple will be very useful for construction of physical maps, for genome sequencing and for gene isolation via map-based cloning in this crop species.

2006Doctoral thesis

Open Access