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Effect of varying dietary levels of LC-PUFA and vegetable oil sources on performance and fatty acids of Senegalese sole post larvae: Puzzling results suggest complete biosynthesis pathway from C18 PUFA to DHA

2014Journal article Restricted access
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dc.contributor.authorNavarro-Guillén, Carmen
dc.contributor.authorEngrola, S.
dc.contributor.authorCastanheira, Maria Filipa
dc.contributor.authorBandarra, N.
dc.contributor.authorHachero-Cruzado, Ismael
dc.contributor.authorTocher, Douglas R.
dc.contributor.authorConceição, L. E. C.
dc.contributor.authorMorais, S.
dc.date.accessioned2014-07-03T08:37:51Z
dc.date.available2014-07-03T08:37:51Z
dc.date.issued2014
dc.date.updated2014-06-27T13:45:51Z
dc.description.abstractLipid nutrition of marine fish larvae has focused on supplying essential fatty acids (EFA) at high levels to meet requirements for survival, growth and development. However, some deleterious effects have been reported suggesting that excessive supply of EFA might result in insufficient supply of energy substrates, particularly in species with lower EFA requirements such as Senegalese sole (Solea senegalensis). This study addressed how the balance between EFA and non-EFA (better energy sources) affects larval performance, body composition and metabolism and retention of DHA, by formulating enrichment emulsions containing two different vegetable oil sources (olive oil or soybean oil) and three DHA levels. DHA positively affected growth and survival, independent of oil source, confirming that for sole post-larvae it is advantageous to base enrichments on vegetable oils supplying higher levels of energy, and supplement these with a DHA-rich oil. In addition, body DHA levels were generally comparable considering the large differences in their dietary supply, suggesting that the previously reported ∆4 fatty acyl desaturase (fad) operates in vivo and that DHA was synthesized at physiologically significant rates through a mechanism involving transcriptional up-regulation of ∆4fad, which was significantly up-regulated in the low DHA treatments. Furthermore, data suggested that DHA biosynthesis may be regulated by an interaction between dietary n − 3 and n − 6 PUFA, as well as by levels of LC-PUFA, and this may, under certain nutritional conditions, lead to DHA production from C18 precursors. The molecular basis of putative fatty acyl ∆5 and ∆6 desaturation activities remains to be fully determined as thorough searches have found only a single (∆4) Fads2-type transcript. Therefore, further studies are required but this might represent a unique activity described within vertebrate fads.por
dc.identifier.citationNavarro-Guillén, C.; Engrola, S.; Castanheira, F.; Bandarra, N.; Hachero-Cruzado, I.; Tocher, D.R.; Conceição, L.E.C.; Morais, S.Effect of varying dietary levels of LC-PUFA and vegetable oil sources on performance and fatty acids of Senegalese sole post larvae: Puzzling results suggest complete biosynthesis pathway from C18 PUFA to DHA, Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology, 167, 1, 51-58, 2014.por
dc.identifier.doihttp://dx.doi.org/ 10.1016/j.cbpb.2013.10.001
dc.identifier.issn1096-4959
dc.identifier.urihttp://hdl.handle.net/10400.1/4627
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.relationGastrointestinal and neuropeptides regulating food intake in fish in response to dietary lipid composition
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S1096495913001620por
dc.subjectDiet energy sourcepor
dc.subjectEssential fatty acidspor
dc.subjectFatty acyl desaturasepor
dc.subjectPUFA biosynthesispor
dc.subjectSolea senegalensispor
dc.titleEffect of varying dietary levels of LC-PUFA and vegetable oil sources on performance and fatty acids of Senegalese sole post larvae: Puzzling results suggest complete biosynthesis pathway from C18 PUFA to DHApor
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleGastrointestinal and neuropeptides regulating food intake in fish in response to dietary lipid composition
oaire.awardURIinfo:eu-repo/grantAgreement/EC/FP7/274184/EU
oaire.citation.endPage58por
oaire.citation.startPage51por
oaire.citation.titleComparative Biochemistry and Physiology - B Biochemistry and Molecular Biologypor
oaire.citation.volume167por
oaire.fundingStreamFP7
person.familyNameNavarro-Guillén
person.familyNameEngrola
person.familyNameCastanheira
person.givenNameCarmen
person.givenNameSofia
person.givenNameMaria Filipa
person.identifier237846
person.identifier.ciencia-id281B-5543-D0F3
person.identifier.ciencia-idA610-29E7-9B83
person.identifier.orcid0000-0001-5490-0328
person.identifier.orcid0000-0002-5244-5541
person.identifier.orcid0000-0001-5901-4077
person.identifier.ridA-2485-2012
person.identifier.scopus-author-id55901593400
person.identifier.scopus-author-id8669620600
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.nameEuropean Commission
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication84423569-1ee0-4ed5-8e95-d5ae51008cd3
relation.isAuthorOfPublication51be5a20-df9c-4a90-85eb-51d779642caa
relation.isAuthorOfPublication478e6c75-7e85-4b20-95f6-486ff4b3ac32
relation.isAuthorOfPublication.latestForDiscovery478e6c75-7e85-4b20-95f6-486ff4b3ac32
relation.isProjectOfPublicationf0b5621e-7e08-4cf3-9a78-46cdd1045a48
relation.isProjectOfPublication.latestForDiscoveryf0b5621e-7e08-4cf3-9a78-46cdd1045a48

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