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Temperature responsiveness of gilthead sea bream bone; an in vitro and in vivo approach

2018Journal article Open Access
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dc.contributor.authorRiera-Heredia, Natalia
dc.contributor.authorMartins, Rute
dc.contributor.authorPatricia Mateus, Ana
dc.contributor.authorCosta, Rita
dc.contributor.authorGisbert, Enric
dc.contributor.authorNavarro, Isabel
dc.contributor.authorGutierrez, Joaquim
dc.contributor.authorPower, Deborah M.
dc.contributor.authorCapilla, Encarnacion
dc.date.accessioned2018-12-07T14:57:53Z
dc.date.available2018-12-07T14:57:53Z
dc.date.issued2018
dc.description.abstractThis study aimed to characterize the molecules involved in osteogenesis in seabream and establish using in vitro/in vivo approaches the responsiveness of selected key genes to temperature. The impact of a temperature drop from 23 to 13 degrees C was evaluated in juvenile fish thermally imprinted during embryogenesis. Both, in vitro/in vivo, Fib1a, appeared important in the first stages of bone formation, and Col1A1, ON and OP, in regulating matrix production and mineralization. OCN mRNA levels were up-regulated in the final larval stages when mineralization was more intense. Moreover, temperature-dependent differential gene expression was observed, with lower transcript levels in the larvae at 18 degrees C relative to those at 22 degrees C, suggesting bone formation was enhanced in the latter group. Results revealed that thermal imprinting affected the long-term regulation of osteogenesis. Specifically, juveniles under the low and low-to-high-temperature regimes had reduced levels of OCN when challenged, indicative of impaired bone development. In contrast, gene expression in fish from the high and high-to-low-temperature treatments was unchanged, suggesting imprinting may have a protective effect. Overall, the present study revealed that thermal imprinting modulates bone development in seabream larvae, and demonstrated the utility of the in vitro MSC culture as a reliable tool to investigate fish osteogenesis.
dc.description.sponsorship"Ministerio de Economia y Competitividad" (MINECO) [BES-2015-074654]; Portuguese Science Foundation (FCT) [SFRH/BPD/111512/2015, SFRH/BD/81625/2011]; MINECO, Spain [AGL2010-17324, AGL2014-57974-R]; "Generalitat de Catalunya" (XRAq); Generalitat de Catalunya [2014SGR-01371]; FCT, Portugal [CCMAR/Multi/04326/2013]; European Union [LIFECYCLE EU-FP7 222719]
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.doi10.1038/s41598-018-29570-9
dc.identifier.issn2045-2322
dc.identifier.urihttp://hdl.handle.net/10400.1/11754
dc.language.isoeng
dc.peerreviewedyes
dc.publisherNature Publishing Group
dc.relationBuilding a biological knowledge-base on fish lifecycles for competitive, sustainable European aquaculture
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectSparus-Aurata
dc.subjectGene-expression
dc.subjectOsteoblast differentiation
dc.subjectCell-lines
dc.subjectHeat-shock
dc.subjectProgressive development
dc.subjectExtracellular-matrix
dc.subjectWinter syndrome
dc.subjectRainbow-trout
dc.subjectTeleost fish
dc.titleTemperature responsiveness of gilthead sea bream bone; an in vitro and in vivo approach
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleBuilding a biological knowledge-base on fish lifecycles for competitive, sustainable European aquaculture
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F04326%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F81625%2F2011/PT
oaire.awardURIinfo:eu-repo/grantAgreement/EC/FP7/222719/EU
oaire.citation.startPage11211
oaire.citation.titleScientific Reports
oaire.citation.volume8
oaire.fundingStream5876
oaire.fundingStreamSFRH
oaire.fundingStreamFP7
person.familyNameCosta
person.familyNameGisbert
person.familyNamePower
person.givenNameRita
person.givenNameEnric
person.givenNameDeborah Mary
person.identifier606918
person.identifier.ciencia-id8219-DFF3-929F
person.identifier.ciencia-id891A-8A44-3CAE
person.identifier.orcid0000-0002-6975-7576
person.identifier.orcid0000-0002-7457-8468
person.identifier.orcid0000-0003-1366-0246
person.identifier.ridO-9136-2018
person.identifier.ridB-3654-2011
person.identifier.scopus-author-id57097731700
person.identifier.scopus-author-id7101806760
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameEuropean Commission
rcaap.rightsopenAccess
rcaap.typearticle
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relation.isAuthorOfPublicationd45a0129-65f8-469d-a8d9-74c1d8c40284
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