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Intestinal response to salinity challenge in the Senegalese sole (Solea senegalensis)

2017-02Journal article Restricted access
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dc.contributor.authorRuiz-Jarabo, I.
dc.contributor.authorBarany, A.
dc.contributor.authorJerez-Cepa, I.
dc.contributor.authorMancera, J. M.
dc.contributor.authorFuentes, Juan
dc.date.accessioned2019-11-20T15:07:51Z
dc.date.available2019-11-20T15:07:51Z
dc.date.issued2017-02
dc.description.abstractFish are continuously forced to actively absorb or expel water and ions through epithelia. Most studies have focused on the gill due to its role in Na+ and Cl- trafficking. However, comparatively few studies have focused on the changing function of the intestine in response to external salinity. Therefore, the present study investigated the main intestinal changes of long-term acclimation of the Senegalese sole (Solea senegalensis) to 5,15, 38 and 55 ppt. Through the measurement of short-circuit current (Isc) in Ussing chambers and biochemical approaches, we described a clear anterior/posterior functional regionalization of the intestine in response to salinity. The use of specific inhibitors in Ussing chamber experiments, revealed that the bumetanide-sensitive Na+/K+/Cl- co-transporters are the main effectors of Cl- uptake in both anterior intestine and rectum. Additionally, the use of the anion exchanger specific inhibitor, DIDS, showed a salinity/region dependency of anion exchanger function. Moreover, we also described ouabain-sensitive Na+/K+-ATPase (NKA) and Bafilomycin Al-sensitive H-ATPase activities (HA), which displayed changes related to salinity and intestinal region. However, the most striking result of the present study is the description of an omeprazole-sensitive H+/K+-ATPase (HKA) in the rectum of Senegalese sole. Its activity was consistently measurable and increased at lower salinities, reaching rates even higher than those of the NKA. Together our results provide new insights into the changing role of the intestine in response to external salinity in teleost fish. The rectal activity of HKA offers an alternative/cooperative mechanism with the HA in the final processing of intestinal water absorption by apical titration of secreted bicarbonate. (C) 2016 Elsevier Inc. All rights reserved.
dc.description.sponsorshipMinistry of Science and Education, Spain [AGL2010-14876]
dc.description.sponsorshipMinistry of Science and Higher Education
dc.description.sponsorshipEuropean Social Funds through the Portuguese National Science Foundation (FCT) [PTDC/MAR-BIO/3811/2012, PTDC/MAR-BIO/3034/2014]
dc.description.sponsorshipPortuguese Foundation for Science and Technology (FCT) [UID/Multi/04326/2013]
dc.identifier.doi10.1016/j.cbpa.2016.11.009
dc.identifier.issn1095-6433
dc.identifier.issn1531-4332
dc.identifier.urihttp://hdl.handle.net/10400.1/13248
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier Science Inc
dc.subjectBream sparus-aurata
dc.subjectMarine teleost fish
dc.subjectSalmon salmo-salar
dc.subjectK+-Atpase activity
dc.subjectAcid-base-balance
dc.subjectSea bream
dc.subjectBicarbonate secretion
dc.subjectWater-absorption
dc.subjectHco3-secretion
dc.subjectRainbow-trout
dc.titleIntestinal response to salinity challenge in the Senegalese sole (Solea senegalensis)
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FMAR-BIO%2F3811%2F2012/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F04326%2F2013/PT
oaire.citation.endPage64
oaire.citation.startPage57
oaire.citation.titleComparative Biochemistry and Physiology A-Molecular & Integrative Physiology
oaire.citation.volume204
oaire.fundingStream3599-PPCDT
oaire.fundingStream5876
person.familyNameRuiz-Jarabo
person.familyNameBarany
person.familyNameFuentes
person.givenNameIgnacio
person.givenNameAndre
person.givenNameJuan
person.identifier392998
person.identifier.ciencia-id421B-E196-2C33
person.identifier.orcid0000-0002-3918-0921
person.identifier.orcid0000-0001-7493-8200
person.identifier.orcid0000-0003-0430-8734
person.identifier.scopus-author-id57209284558
person.identifier.scopus-author-id7201832526
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccess
rcaap.typearticle
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