Repository logo
 
Publication

Kisspeptin influences the reproductive axis and circulating levels of microRNAs in Senegalese sole

2020Journal article Open access
Simple item page
dc.contributor.authorOliveira, Catarina
dc.contributor.authorFatsini, Elvira
dc.contributor.authorFernández, Ignacio
dc.contributor.authorAnjos, Catarina
dc.contributor.authorChauvigné, François
dc.contributor.authorCerdà, Joan
dc.contributor.authorMjelle, Robin
dc.contributor.authorFernandes, Jorge M. O.
dc.contributor.authorCabrita, Elsa
dc.date.accessioned2021-01-11T18:13:53Z
dc.date.available2021-01-11T18:13:53Z
dc.date.issued2020
dc.description.abstractKisspeptin regulates puberty and reproduction onset, acting upstream of the brain-pituitary-gonad (HPG) axis. This study aimed to test a kisspeptin-based hormonal therapy on cultured Senegalese sole (G1) breeders, known to have reproductive dysfunctions. A single intramuscular injection of KISS2-10 decapeptide (250 µg/kg) was tested in females and males during the reproductive season, and gonad maturation, sperm motility, plasma levels of gonadotropins (Fsh and Lh) and sex steroids (11-ketotestosterone, testosterone and estradiol), as well as changes in small non-coding RNAs (sncRNAs) in plasma, were investigated. Fsh, Lh, and testosterone levels increased after kisspeptin injection in both sexes, while sperm analysis did not show differences between groups. Let7e, miR-199a-3p and miR-100-5p were differentially expressed in females, while miR-1-3p miRNA was up-regulated in kisspeptin-treated males. In silico prediction of mRNAs targeted by miRNAs revealed that kisspeptin treatment might affect paracellular transporters, regulate structural and functional polarity of cells, neural networks and intracellular trafficking in Senegalese sole females; also, DNA methylation and sphingolipid metabolism might be altered in kisspeptin-treated males. Results demonstrated that kisspeptin stimulated gonadotropin and testosterone secretion in both sexes and induced an unanticipated alteration of plasma miRNAs, opening new research venues to understand how this neuropeptide impacts in fish HPG axis.pt_PT
dc.description.sponsorshipProject ReproF1 (Programa Operacional Mar2020, MAR-16-02-01-FMP-0059 to E.C.), FCT: UIDB/04326/2020/ DL 57/2016/CP1361/CT0007 and SFRH/BD/130910/2017. COST O ce (COST Action 16120: EPITRANS)pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.3390/ijms21239051pt_PT
dc.identifier.eissn1422-0067
dc.identifier.urihttp://hdl.handle.net/10400.1/14947
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationINNOVATIVE EPIGENETIC MARKERS FOR FISH DOMESTICATION
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectSolea senegalensispt_PT
dc.subjectSex steroidspt_PT
dc.subjectHormonal treatmentpt_PT
dc.subjectKISS2pt_PT
dc.subjectG1 breederspt_PT
dc.subjectGonadotropinspt_PT
dc.titleKisspeptin influences the reproductive axis and circulating levels of microRNAs in Senegalese solept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleINNOVATIVE EPIGENETIC MARKERS FOR FISH DOMESTICATION
oaire.awardURIinfo:eu-repo/grantAgreement/EC/H2020/683210/EU
oaire.citation.issue23pt_PT
oaire.citation.startPage9051pt_PT
oaire.citation.titleInternational Journal of Molecular Sciencespt_PT
oaire.citation.volume21pt_PT
oaire.fundingStreamH2020
person.familyNameOliveira
person.familyNameFatsini Fernández
person.familyNameAnjos
person.familyNameCabrita
person.givenNameCatarina
person.givenNameElvira
person.givenNameCatarina
person.givenNameElsa
person.identifierhttps://scholar.google.es/citations?hl=en&pli=1&user=55mbsZIAAAAJ
person.identifier.ciencia-id0816-30C5-51AC
person.identifier.ciencia-idEA1D-0571-7A6D
person.identifier.ciencia-id3B1A-C5A1-E96C
person.identifier.orcid0000-0002-6679-761X
person.identifier.orcid0000-0003-0371-5290
person.identifier.orcid0000-0002-2038-2187
person.identifier.orcid0000-0003-3864-8841
person.identifier.ridE-8523-2012
person.identifier.scopus-author-id35433190500
person.identifier.scopus-author-id26645697000
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.nameEuropean Commission
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication3ac5841f-3b4e-4ad1-94f7-a5436fb8cd59
relation.isAuthorOfPublicationde251b78-72d6-49ee-bcc2-79db8f64efb8
relation.isAuthorOfPublicationacc899b9-bc7a-4e9c-a7ad-1226c32c5da3
relation.isAuthorOfPublication332fa9b9-09b5-47fe-a1b3-afe088b4150a
relation.isAuthorOfPublication.latestForDiscoveryacc899b9-bc7a-4e9c-a7ad-1226c32c5da3
relation.isProjectOfPublication3192cddc-26ed-4d48-903d-5039486fec0f
relation.isProjectOfPublication.latestForDiscovery3192cddc-26ed-4d48-903d-5039486fec0f

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
ijms-21-09051.pdf
Size:
2.97 MB
Format:
Adobe Portable Document Format
Download

Collections

CCM2-Artigos (em revistas ou actas indexadas)