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Docosahexaenoic acid loaded lipid nanoparticles with bactericidal activity against Helicobacter pylon

2017-03Journal article Restricted access
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dc.contributor.authorSeabra, Catarina Leal
dc.contributor.authorNunes, Claudia
dc.contributor.authorGomez-Lazaro, Maria
dc.contributor.authorCorreia, Maria Marta
dc.contributor.authorMachado, José Carlos
dc.contributor.authorGonçalves, Inês C.
dc.contributor.authorReis, Celso A.
dc.contributor.authorReis, Salette
dc.contributor.authorMartins, M. Cristina L.
dc.date.accessioned2019-11-20T15:07:43Z
dc.date.available2019-11-20T15:07:43Z
dc.date.issued2017-03
dc.description.abstractDocosahexaenoic acid (DHA), an omega-3 polyunsaturated fatty acid present in fish oil, has been described as a promising molecule to the treatment of Helicobacter pylori gastric infection. However, due to its highly unsaturated structure, DHA can be easily oxidized loosing part of its bioactivity. This work aims the nanoencapsulation of DHA to improve its bactericidal efficacy against H. pylori. DHA was loaded into nanostructured lipid carriers (NLC) produced by hot homogenization and ultrasonication using a blend of lipids (Precirol AT05 (R), Miglyol-812 (R)) and a surfactant (Tween 60 (R)). Homogeneous NLC with 302 +/- 14 nm diameter,-28 +/- 3 mV surface charge (dynamic and electrophoretic light scattering) and containing 66 +/- 7% DHA (UV/VIS spectroscopy) were successfully produced. Bacterial growth curves, performed over 24 h in the presence of different DHA concentrations (free or loaded into NLC), demonstrated that nanoencapsulation enhanced DHA bactericidal effect, since DHA-loaded NLC were able to inhibit H. pylori growth in a much lower concentrations (25 mu M) than free DHA (>100 mu M). Bioimaging studies, using scanning and transmission electron microscopy and also imaging flow cytometry, demonstrated that DHA-loaded NLC interact with H. pylori membrane, increasing their periplasmic space and disrupting membrane and allowing the leakage of cytoplasmic content. Furthermore, the developed nanoparticles are not cytotoxic to human gastric adenocarcinoma cells at bactericidal concentrations. DHA-loaded NLC should, therefore, be envisaged as an alternative to the current treatments for H. pylori infection. (C) 2017 Elsevier B.V. All rights reserved.
dc.description.sponsorshipFCT/MCTES [POCI-01-0145-FEDER-007274, PTDC/CTM-BIO/4043/2014, PTDC/CTM-BPC/121149/2010, NORTE-01-0145-FEDER-000012]
dc.description.sponsorshipFEDER funds through the COMPETE (POCI), Portugal
dc.description.sponsorshipFCT grant [SFRH/BD/89001/2012]
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.doi10.1016/j.ijpharm.2017.01.014
dc.identifier.issn0378-5173
dc.identifier.issn1873-3476
dc.identifier.urihttp://hdl.handle.net/10400.1/13183
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier Science Bv
dc.subjectPolyunsaturated fatty-acids
dc.subjectGastric-cancer cells
dc.subjectOral bioavailability
dc.subjectDrug-delivery
dc.subjectCarriers nlc
dc.subjectIn-vitro
dc.subjectInfection
dc.subjectSystems
dc.subjectMonoglycerides
dc.subjectEradication
dc.titleDocosahexaenoic acid loaded lipid nanoparticles with bactericidal activity against Helicobacter pylon
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/COMPETE/PTDC%2FCTM-BPC%2F121149%2F2010/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F89001%2F2012/PT
oaire.citation.endPage137
oaire.citation.issue01-fev
oaire.citation.startPage128
oaire.citation.titleInternational Journal of Pharmaceutics
oaire.citation.volume519
oaire.fundingStreamCOMPETE
oaire.fundingStreamSFRH
person.familyNameCorreia
person.givenNameMarta
person.identifier2441460
person.identifier.ciencia-id401F-A7D3-4B0C
person.identifier.orcid0000-0001-5040-735X
person.identifier.scopus-author-id26648073400
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccess
rcaap.typearticle
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relation.isAuthorOfPublication.latestForDiscoveryafd72799-3911-445a-ba29-772737a2433e
relation.isProjectOfPublication607c7452-2faf-435e-9cf4-47c395bcb3a2
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