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Quantitation overcoming Matrix effects of Lipophilic toxins in Mytilus galloprovincialis by liquid chromatography-full scan high resolution mass spectrometry analysis (LC-HR-MS)

2022-02-15Journal article Open access
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dc.contributor.authorCosta, Camila Q. V. da
dc.contributor.authorAfonso, Inês I.
dc.contributor.authorLage, Sandra
dc.contributor.authorCosta, Pedro Reis
dc.contributor.authorCanario, Adelino
dc.contributor.authorSilva, José Paulo da
dc.date.accessioned2022-03-09T15:03:18Z
dc.date.available2022-03-09T15:03:18Z
dc.date.issued2022-02-15
dc.date.updated2022-02-24T14:50:12Z
dc.description.abstractThe analysis of marine lipophilic toxins in shellfish products still represents a challenging task due to the complexity and diversity of the sample matrix. Liquid chromatography coupled with mass spectrometry (LC-MS) is the technique of choice for accurate quantitative measurements in complex samples. By combining unambiguous identification with the high selectivity of tandem MS, it provides the required high sensitivity and specificity. However, LC-MS is prone to matrix effects (ME) that need to be evaluated during the development and validation of methods. Furthermore, the large sample-to-sample variability, even between samples of the same species and geographic origin, needs a procedure to evaluate and control ME continuously. Here, we analyzed the toxins okadaic acid (OA), dinophysistoxins (DTX-1 and DTX-2), pectenotoxin (PTX-2), yessotoxin (YTX) and azaspiracid-1 (AZA-1). Samples were mussels (<i>Mytilus galloprovincialis</i>), both fresh and processed, and a toxin-free mussel reference material. We developed an accurate mass-extracted ion chromatogram (AM-XIC) based quantitation method using an Orbitrap instrument, evaluated the ME for different types and extracts of mussel samples, characterized the main compounds co-eluting with the targeted molecules and quantified toxins in samples by following a standard addition method (SAM). An AM-XIC based quantitation of lipophilic toxins in mussel samples using high resolution and accuracy full scan profiles (LC-HR-MS) is a good alternative to multi reaction monitoring (MRM) for instruments with HR capabilities. ME depend on the starting sample matrix and the sample preparation. ME are particularly strong for OA and related toxins, showing values below 50% for fresh mussel samples. Results for other toxins (AZA-1, YTX and PTX-2) are between 75% and 110%. ME in unknown matrices can be evaluated by comparing their full scan LC-HR-MS profiles with those of known samples with known ME. ME can be corrected by following SAM with AM-XIC quantitation if necessary.pt_PT
dc.description.sponsorshipEMBRC.PT ALG-01-0145-FEDER-022121
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifierdoi: 10.3390/md20020143
dc.identifier.citationMarine Drugs 20 (2): 143 (2022)pt_PT
dc.identifier.doi10.3390/md20020143pt_PT
dc.identifier.issn1660-3397
dc.identifier.urihttp://hdl.handle.net/10400.1/17656
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationAlgarve Centre for Marine Sciences
dc.relationEmergent toxins on the Portuguese coast: occurrence, transfer kinetics and toxicity
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectLiquid chromatography-mass spectrometry (LC-MS)pt_PT
dc.subjectLiquid chromatography-high resolution mass spectrometry (LC-HR-MS)pt_PT
dc.subjectFull scanpt_PT
dc.subjectMatrix effectspt_PT
dc.subjectOkadaic acid (OA)pt_PT
dc.subjectDinophysistoxin-1 (DTX-1)pt_PT
dc.subjectDinophysistoxin-2 (DTX-2)pt_PT
dc.subjectPectenotoxin-2 (PTX-2)pt_PT
dc.subjectAzaspiracid-1 (AZA-1)pt_PT
dc.subjectYessotoxin (YTX)pt_PT
dc.titleQuantitation overcoming Matrix effects of Lipophilic toxins in Mytilus galloprovincialis by liquid chromatography-full scan high resolution mass spectrometry analysis (LC-HR-MS)pt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardTitleEmergent toxins on the Portuguese coast: occurrence, transfer kinetics and toxicity
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04326%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/EC/H2020/101003376/EU
oaire.citation.issue2pt_PT
oaire.citation.startPage143pt_PT
oaire.citation.titleMarine Drugspt_PT
oaire.citation.volume20pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamH2020
person.familyNameQ. V. da Costa
person.familyNameLage
person.familyNameReis Costa
person.familyNameCanario
person.familyNameSilva
person.givenNameCamila
person.givenNameSandra
person.givenNamePedro
person.givenNameAdelino
person.givenNameJosé Paulo da
person.identifier2065549
person.identifier454970
person.identifier600820
person.identifier143624
person.identifier.ciencia-id931B-9699-7396
person.identifier.ciencia-idDA15-C119-BDC8
person.identifier.ciencia-idC911-9715-E547
person.identifier.ciencia-id1F1E-D3B3-F804
person.identifier.ciencia-id3413-C4F4-73F7
person.identifier.orcid0000-0001-8725-9100
person.identifier.orcid0000-0003-0167-7163
person.identifier.orcid0000-0001-6083-470X
person.identifier.orcid0000-0002-6244-6468
person.identifier.orcid0000-0002-6458-7328
person.identifier.ridN-1908-2019
person.identifier.ridC-7942-2009
person.identifier.ridA-4606-2008
person.identifier.scopus-author-id54585529500
person.identifier.scopus-author-id7201895802
person.identifier.scopus-author-id56568523700
person.identifier.scopus-author-id7201733236
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameEuropean Commission
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublicatione2004fa2-a47d-44b0-b11c-fc95fe51cbf9
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