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Evolutionary history and functional characterization of duplicated G protein-coupled estrogen receptors in European sea bass

2024Journal article Open access
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dc.contributor.authorZapater, Cinta
dc.contributor.authorMoreira, Catarina
dc.contributor.authorKnigge, Thomas
dc.contributor.authorMonsinjon, Tiphaine
dc.contributor.authorGómez, Ana
dc.contributor.authorPinto, Patricia I. S.
dc.date.accessioned2024-03-13T10:37:28Z
dc.date.available2024-03-13T10:37:28Z
dc.date.issued2024
dc.description.abstractAcross vertebrates, the numerous estrogenic functions are mainly mediated by nuclear and membrane receptors, including the G protein-coupled estrogen receptor (GPER) that has been mostly associated with rapid non-genomic responses. Although Gper-mediated signalling has been characterized in only few fish species, Gpers in fish appear to present more mechanistic functionalities as those of mammals due to additional gene duplicates. In this study, we ran a thorough investigation of the fish Gper evolutionary history in light of available genomes, we carried out the functional characterization of the two gper gene duplicates of European sea bass (Dicentrarchus labrax) using luciferase reporter gene transactivation assays, validated it with natural and synthetic estrogen agonists/antagonists and applied it to other chemicals of aquaculture and ecotoxicological interest. Phylogenetic and synteny analyses of fish gper1 and gper1-like genes suggest their duplication may have not resulted from the teleost-specific whole genome duplication. We confirmed that both sbsGper isoforms activate the cAMP signalling pathway and respond differentially to distinct estrogenic compounds. Therefore, as observed for nuclear estrogen receptors, both sbsGpers duplicates retain estrogenic activity although they differ in their specificity and potency (Gper1 being more potent and more specific than Gper1-like), suggesting a more conserved role for Gper1 than for Gper1-like. In addition, Gpers were able to respond to estrogenic environmental pollutants known to interfere with estrogen signalling, such as the phytoestrogen genistein and the anti-depressant fluoxetine, a point that can be taken into account in aquatic environment pollution screenings and chemical risk assessment, complementing previous assays for sea bass nuclear estrogen receptors.pt_PT
dc.description.sponsorshipETaT (ANR-15-CE32-0014); MCIN/AEI/10.13039/501100011033; AGL2015-67477-C2-1-R; PID2021-122929OB-C32; "PHC PESSOA” program (project number: 44642RM);pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.jsbmb.2023.106423pt_PT
dc.identifier.issn0960-0760
dc.identifier.urihttp://hdl.handle.net/10400.1/20498
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationAlgarve Centre for Marine Sciences
dc.relationAlgarve Centre for Marine Sciences
dc.relationCentre for Marine and Environmental Research
dc.relationNot Available
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subject17β-Estradiolpt_PT
dc.subjectGenisteinpt_PT
dc.subjectFluoxetinept_PT
dc.subjectTeleost fishpt_PT
dc.subjectTransactivation assaypt_PT
dc.subjectEndocrine disruptionpt_PT
dc.titleEvolutionary history and functional characterization of duplicated G protein-coupled estrogen receptors in European sea basspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardTitleCentre for Marine and Environmental Research
oaire.awardTitleNot Available
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04326%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04326%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0101%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/DL 57%2F2016/DL 57%2F2016%2FCP1361%2FCT0015/PT
oaire.citation.startPage106423pt_PT
oaire.citation.titleThe Journal of Steroid Biochemistry and Molecular Biologypt_PT
oaire.citation.volume236pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamDL 57/2016
person.familyNamePinto
person.givenNamePatricia IS
person.identifier643457
person.identifier.ciencia-idE51D-5CCB-B1C6
person.identifier.orcid0000-0001-7854-3898
person.identifier.ridM-3817-2013
person.identifier.scopus-author-id10240774300
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication64bc526e-5281-44e5-91eb-5743436109f9
relation.isAuthorOfPublication.latestForDiscovery64bc526e-5281-44e5-91eb-5743436109f9
relation.isProjectOfPublicationfafa76a6-2cd2-4a6d-a3c9-772f34d3b91f
relation.isProjectOfPublication15f91d45-e070-47d8-b6b8-efd4de31d9a8
relation.isProjectOfPublication794d4c77-c731-471e-bc96-5a41dcd3d872
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