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Desenvolvimento de sistemas celulares de peixe adequados ao estudo da mineralização in vitro

2009-09Journal article Open access
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dc.contributor.authorMarques, C. L.
dc.contributor.authorRafael, Marta S.
dc.contributor.authorTiago, Daniel
dc.contributor.authorCancela, Leonor
dc.contributor.authorLaizé, Vincent
dc.date.accessioned2014-06-12T14:40:56Z
dc.date.available2014-06-12T14:40:56Z
dc.date.issued2009-09
dc.date.updated2014-06-03T10:17:46Z
dc.description.abstractOs peixes foram recentemente reconhecidos como organismos modelo apropriados para o estudo da biologia de vertebrados, particularmente de processos relacionados com a mineralização tecidular e o desenvolvimento do esqueleto. Apesar de existirem alguns estudos in vivo, a identificação de mecanismos associados à calcificação em peixes tem sido prejudicada pelo facto de não existirem sistemas celulares para estudos in vitro. Este artigo descreve um protocolo simples e de baixo custo adequado ao desenvolvimento de culturas celulares mineralogénicas, derivadas de tecidos calcificados de peixes.por
dc.description.abstractFish have been recently recognized as a suitable model organism to study vertebrate biology, in particular physiological processes such as tissue mineralization and skeletal development. Despite various studies in vivo, identification of mechanisms associated with calcification in fish has been largely hampered by the lack of suitable in vitro cell systems. We describe here a simple and inexpensive protocol to develop mineralogenic cell cultures from fish calcified tissues. This protocol was successfully used to develop the first fish cell lines capable of mineralizing their extracellular matrix (from the vertebra of gilthead seabream Sparus aurata) and, more recently, applied to develop additional fish mineralogenic cell cultures from different fishes. We also describe a variety of biochemical and histological methods to characterize extracellular matrix during in vitro mineralization, in particular mineral deposition, and a protocol to enhance DNA transfer into fish bone-derived cells. Finally, we present recent expression data obtained using these cell lines to further demonstrate their suitability to study mechanisms of in vitro mineralization.por
dc.identifier.citationMarques CL; Rafael MS; Tiago DM; Cancela ML; Laizé, Vincent. Desenvolvimento de sistemas celulares de peixe adequados ao estudo da mineralização in vitro, Canal BQ, 01/05/2009, 15-20.por
dc.identifier.issn1646-4192
dc.identifier.otherAUT: LCA00739
dc.identifier.urihttp://hdl.handle.net/10400.1/4289
dc.language.isoporpor
dc.peerreviewedyespor
dc.publisherSociedade Portuguesa de Bioquímicapor
dc.relation.publisherversionhttp://www.canalbq.spb.pt/docs/canalBQ_0006.pdfpor
dc.subjectSistemas celulares in vitropor
dc.subjectMineralizaçãopor
dc.subjectTecidos calcificadospor
dc.subjectDourada (Sparus aurata L.)por
dc.titleDesenvolvimento de sistemas celulares de peixe adequados ao estudo da mineralização in vitropor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage20por
oaire.citation.issue6por
oaire.citation.startPage15por
oaire.citation.titleCanalBQpor
person.familyNameRafael
person.familyNameTiago
person.familyNameCancela
person.familyNameLaizé
person.givenNameMarta
person.givenNameDaniel
person.givenNameM. Leonor
person.givenNameVincent
person.identifier.ciencia-id1C10-BF17-0DD7
person.identifier.orcid0000-0002-5518-9790
person.identifier.orcid0000-0001-8418-6292
person.identifier.orcid0000-0003-3114-6662
person.identifier.orcid0000-0001-9565-9198
person.identifier.ridB-4463-2008
person.identifier.scopus-author-id15078212800
person.identifier.scopus-author-id14422711000
person.identifier.scopus-author-id6602982778
rcaap.rightsopenAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublicatione4c6f8da-c3f6-4eee-bf78-f023843c343b
relation.isAuthorOfPublication10cda3a0-7b9c-4d16-bd2a-a06df1d56d94
relation.isAuthorOfPublicationb9bbfe32-3dfe-4131-ad14-a4394008447f
relation.isAuthorOfPublicationb51cd3d5-7ee4-47d8-9bc4-b3e297aa222f
relation.isAuthorOfPublication.latestForDiscovery10cda3a0-7b9c-4d16-bd2a-a06df1d56d94

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