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High rates of intestinal bicarbonate secretion in seawater tilapia (Oreochromis mossambicus)

2017-05Journal article Restricted access
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dc.contributor.authorRuiz-Jarabo, I.
dc.contributor.authorGregorio, Silvia F.
dc.contributor.authorGaetano, P.
dc.contributor.authorTrischitta, F.
dc.contributor.authorFuentes, Xoan
dc.date.accessioned2019-11-20T15:07:38Z
dc.date.available2019-11-20T15:07:38Z
dc.date.issued2017-05
dc.description.abstractOsmoregulation in fish is a complex process that requires the orchestrated cooperation of many tissues. In fish facing hyperosmotic environments, the intestinal absorption of some monovalent ions and the secretion of bicarbonate are key processes to favor water absorption. In the present study, we showed that bicarbonate levels in the intestinal fluid are several fold higher in seawater than in freshwater acclimated tilapia (Oreochromis mossambicus). In addition, we analyzed gene expression of the main molecular mechanisms involved in HCO3- movements i.e. slc26a6, slc26a3, slc4a4 and v-type H-ATPase sub C in the intestine of tilapia acclimated to both seawater and freshwater. Our results show an anterior/posterior functional regionalization of the intestine in tilapia in terms of expression patterns, which is affected by environmental salinity mostly in the anterior and mid intestine. Analysis of bicarbonate secretion using pH-Stat in tissues mounted in Ussing chambers reveals high rates of bicarbonate secretion in tilapia acclimated to seawater from anterior intestine to rectum ranging between similar to 900 and similar to 1700 nmol HCO3- cm(-2) h(-1). However, a relationship between the expression of slc26a6, slc26a3, slc4a4 and the rate of bicarbonate secretion seems to be compromised in the rectum. In this region, the low expression of the bicarbonate transporters could not explain the high bicarbonate secretion rates here described. However, we postulate that the elevated v-type H-ATPase mRNA expression in the rectum could be involved in this process. (C) 2017 Elsevier Inc. All rights reserved.
dc.description.sponsorshipUniversity of Cadiz, Spain
dc.description.sponsorshipFCT, Portugal [SFRH/BD/113363/2015]
dc.description.sponsorshipPortuguese Foundation for Science and Technology (Ministry of Science and Higher Education, Portugal) [PTDC/MAR-BIO/3034/2014]
dc.description.sponsorshipPortuguese Foundation for Science and Technology (European Social Funds) [PTDC/MAR-BIO/3034/2014]
dc.description.sponsorshipnational funds from the Portuguese Foundation for Science and Technology (FCT) [UID/Multi/04326/2013]
dc.identifier.doi10.1016/j.cbpa.2017.02.022
dc.identifier.issn1095-6433
dc.identifier.issn1531-4332
dc.identifier.urihttp://hdl.handle.net/10400.1/13143
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier Science Inc
dc.subjectMarine teleost fish
dc.subjectBream sparus-aurata
dc.subjectK+-Atpase activity
dc.subjectAcid-base-balance
dc.subjectSea bream
dc.subjectWater-absorption
dc.subjectHco3-secretion
dc.subjectRainbow-trout
dc.subjectCarbonic-anhydrase
dc.subjectFundulus-heteroclitus
dc.titleHigh rates of intestinal bicarbonate secretion in seawater tilapia (Oreochromis mossambicus)
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage64
oaire.citation.startPage57
oaire.citation.titleComparative Biochemistry and Physiology - Part A
oaire.citation.volume207
person.familyNameRuiz-Jarabo
person.familyNameGregorio
person.familyNameFuentes
person.givenNameIgnacio
person.givenNameSilvia
person.givenNameJuan
person.identifier1579837
person.identifier392998
person.identifier.ciencia-idBF14-020B-4C39
person.identifier.ciencia-id421B-E196-2C33
person.identifier.orcid0000-0002-3918-0921
person.identifier.orcid0000-0002-6648-5122
person.identifier.orcid0000-0003-0430-8734
person.identifier.ridG-1618-2018
person.identifier.scopus-author-id7201832526
rcaap.rightsrestrictedAccess
rcaap.typearticle
relation.isAuthorOfPublication31cbd691-c8ea-4ad5-838e-bcf58a4f533f
relation.isAuthorOfPublication9bc940d8-7dfb-4950-98f4-6853f398cb49
relation.isAuthorOfPublicationd354b937-b18c-4ca5-a843-04a04269d60f
relation.isAuthorOfPublication.latestForDiscoveryd354b937-b18c-4ca5-a843-04a04269d60f

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