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Isolation, identification and biotechnological applications of a novel, robust, free-living Chlorococcum (Oophila) amblystomatis strain isolated from a local pond

2020Journal article Open access
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dc.contributor.authorCorreia, Nádia
dc.contributor.authorPereira, Hugo
dc.contributor.authorSilva, Joana T.
dc.contributor.authorSantos, Tamara
dc.contributor.authorSoares, Maria
dc.contributor.authorSousa, Carolina B.
dc.contributor.authorSchüler, Lisa Maylin
dc.contributor.authorCosta, Margarida
dc.contributor.authorJ. C. or Varela J. or Varela J.C.S.
dc.contributor.authorPereira, Leonel
dc.contributor.authorSilva, Joana
dc.date.accessioned2020-06-16T10:42:24Z
dc.date.available2020-06-16T10:42:24Z
dc.date.issued2020
dc.description.abstractBioprospection of novel autochthonous strains is key to the successful industrial-scale production of microalgal biomass. A novel Chlorococcum strain was recently isolated from a pond inside the industrial production facility of Allmicroalgae (Leiria, Portugal). Phylogenetic analysis based on 18S ribosomal ribonucleic acid (rRNA) gene sequences suggests that this isolate is a novel, free-living Oophila amblystomatis strain. However, as our phylogenetic data strongly suggests that the aforementioned taxon belongs to the genus Chlorococcum, it is here proposed to rename this species as Chlorococcum amblystomatis. In order to characterize the biotechnological potential of this novel isolate, growth performance and biochemical composition were evaluated from the pilot (2.5-m3) to industrial (10-m3) scale. The highest maximum areal productivity (36.56 g m􀀀2 day􀀀1) was reached in a 10-m3 tubular photobioreactor (PBR), as compared to that obtained in a 2.5-m3 PBR (26.75 g m􀀀2 day􀀀1). Chlorococcum amblystomatis displayed high protein content (48%–56% dry weight (DW)) and moderate levels of total lipids (18%–31% DW), carbohydrates (6%–18% DW) and ashes (9%–16% DW). Furthermore, the lipid profile was dominated by polyunsaturated fatty acids (PUFAs). The highest pigment contents were obtained in the 2.5-m3 PBR, where total chlorophylls accounted for 40.24 mg g􀀀1 DW, followed by lutein with 5.37 mg g􀀀1 DW. Overall, this free-living Chlorococcum amblystomatis strain shows great potential for nutritional applications, coupling a promising growth performance with a high protein content as well as relevant amounts of PUFAs, chlorophyll, and carotenoids.pt_PT
dc.description.sponsorshipFCT: UIDB/04326/2020, UIDB/04292/2020. Projects EMBRC.PT ALG-01-0145-FEDER-022121 and BIODATA.PT ALG-01-0145-FEDER-02223.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.3390/app10093040pt_PT
dc.identifier.issn2076-3417
dc.identifier.urihttp://hdl.handle.net/10400.1/14017
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectBioprospectionpt_PT
dc.subjectBiotechnological applicationspt_PT
dc.subjectMicroalgaept_PT
dc.subjectIndustrial-scale production;pt_PT
dc.subjectChlorococcum (Oophila) amblystomatispt_PT
dc.subjectPhotobioreactorspt_PT
dc.titleIsolation, identification and biotechnological applications of a novel, robust, free-living Chlorococcum (Oophila) amblystomatis strain isolated from a local pondpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue9pt_PT
oaire.citation.startPage3040pt_PT
oaire.citation.titleApplied Sciencespt_PT
oaire.citation.volume10pt_PT
person.familyNamePereira
person.familyNameSantos
person.familyNameSchüler
person.familyNameVarela
person.givenNameHugo
person.givenNameTamara
person.givenNameLisa
person.givenNameJoão
person.identifier282781
person.identifier.ciencia-id331E-84FD-2F34
person.identifier.ciencia-idE01F-9B43-4F24
person.identifier.orcid0000-0002-1369-2099
person.identifier.orcid0000-0001-9008-4436
person.identifier.orcid0000-0003-0961-5397
person.identifier.orcid0000-0003-3101-693X
person.identifier.ridE-9360-2015
person.identifier.ridM-4223-2013
person.identifier.scopus-author-id54080259900
person.identifier.scopus-author-id55741416000
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublication6011d40b-5657-4f76-8bb5-37f359562a11
relation.isAuthorOfPublication7311726c-0c21-4c6b-bd6f-d3f01d0a5a62
relation.isAuthorOfPublicationd339a9e7-bc32-49c4-ba48-a17343d40092
relation.isAuthorOfPublication.latestForDiscoveryebcb2180-5235-4502-b562-cd1e7786e6f9

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