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Synchronous insight of in vitro and in vivo biological activities of Sambucus nigra L. extracts for industrial uses

2020-10Journal article Restricted access
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dc.contributor.authorMota, Ana Henriques
dc.contributor.authorAndrade, Joana M.
dc.contributor.authorRodrigues, Maria Joao
dc.contributor.authorCustódio, Luísa
dc.contributor.authorBronze, Maria Rosario
dc.contributor.authorDuarte, Noelia
dc.contributor.authorBaby, Andre
dc.contributor.authorRocha, Joao
dc.contributor.authorGaspar, Maria Manuela
dc.contributor.authorSimoes, Sandra
dc.contributor.authorCarvalheiro, Manuela
dc.contributor.authorFattal, Elias
dc.contributor.authorAlmeida, Antonio Jose
dc.contributor.authorReis, Catarina Pinto
dc.date.accessioned2021-06-24T11:35:10Z
dc.date.available2021-06-24T11:35:10Z
dc.date.issued2020-10
dc.description.abstractThere is a re-emerging interest in natural products as reputable sources of new active pharmaceutical ingredients. This study synchronously reports in vitro, with more than one cell line, and in vivo biological activities of extracts obtained from Sambucus nigra. Using several solvents and techniques, eighteen extracts were obtained from fresh and dried berries, and fresh flowers. The flavonoid content and identification were determined using HPLC-MS/MS. The extracts were then screened for antioxidant activity, total polyphenol content, collagenase, elastase, tyrosinase and acetylcholinesterase inhibition as well as photoprotection. In vitro and in vivo (murine model) anti-inflammatory activity and cytotoxicity (skin and monocytic cells) were also studied. The most promising extracts were those obtained from fresh flowers using either ultrasounds or methanol. These extracts showed similar results to positive controls, particularly the antioxidant activity (74.5 +/- 1.6 %), collagenase inhibition (93.6 +/- 0.6 %), photoprotection (Sun Protection Factor > 50), in vitro anti-inflammatory activity (96.9 +/- 2.9 %), as well as oral/topical anti-inflammatory activity. The ultrasounds/ethanol extract of fresh flowers presented higher collagenase inhibition (88.3 +/- 2.8 %) and in vitro anti-inflammatory activity (101.8 +/- 1.5 %). Cytotoxicity testing confirmed the safety. Chemical characterization allowed the deduction of a correlation between extract composition and biological activities, suggesting a straightforward application in the development of novel products subject to further investigation.
dc.description.sponsorshipFundacao para a Ciencia e a TecnologiaPortuguese Foundation for Science and TechnologyEuropean Commission [UID/DTP/04138/2019]
dc.description.sponsorshipPortugal 2020 [LISBOA-01-0145-FEDER-402-022125]
dc.description.sponsorshipPortuguese National and Regional Budget [CCMAR/Multi/04326/2019]
dc.description.sponsorshipFCTPortuguese Foundation for Science and TechnologyEuropean Commission [SFRH/BD/116604/2016]
dc.description.sponsorshipFCT Scientific Employment Stimulus [CEECIND/00425/2017]
dc.identifier.doi10.1016/j.indcrop.2020.112709
dc.identifier.issn0926-6690
dc.identifier.urihttp://hdl.handle.net/10400.1/16361
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier
dc.relationUnravelling the biotechnological potential of halophyte species from the Algarve coast - HaloBiotech
dc.relationResearch Institute for Medicines
dc.subjectSambucus extracts
dc.subjectAntioxidant activity
dc.subjectCollagenase inhibition
dc.subjectAnti-inflammatory activities
dc.subjectPhotoprotection efficiency
dc.subjectCytotoxicity
dc.subject.otherAgriculture
dc.titleSynchronous insight of in vitro and in vivo biological activities of Sambucus nigra L. extracts for industrial uses
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleUnravelling the biotechnological potential of halophyte species from the Algarve coast - HaloBiotech
oaire.awardTitleResearch Institute for Medicines
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/SFRH%2FBD%2F116604%2F2016/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FDTP%2F04138%2F2019/PT
oaire.citation.startPage112709
oaire.citation.titleIndustrial Crops and Products
oaire.citation.volume154
oaire.fundingStreamOE
oaire.fundingStream6817 - DCRRNI ID
person.familyNameRodrigues
person.familyNameCustódio
person.givenNameMaria João
person.givenNameLuísa
person.identifierR-004-VNG
person.identifier.ciencia-id2514-0E17-1D8D
person.identifier.ciencia-id791B-C560-AEA2
person.identifier.orcid0000-0001-8732-710X
person.identifier.orcid0000-0003-4338-7703
person.identifier.ridM-6101-2013
person.identifier.scopus-author-id56031608100
person.identifier.scopus-author-id15831018900
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccess
rcaap.typearticle
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relation.isAuthorOfPublicationf9cfed0f-6b67-413e-988c-ac7397183471
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relation.isProjectOfPublication31fb3213-3ff4-489c-bce2-9d6289e46c08
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