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Solea senegalensis sperm cryopreservation: new insights on sperm quality

2017-10Journal article Open Access
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dc.contributor.authorF RIESCO, MARTA
dc.contributor.authorOliveira, Catarina
dc.contributor.authorSoares, Florbela
dc.contributor.authorGavaia, Paulo J.
dc.contributor.authorDinis, Maria Teresa
dc.contributor.authorCabrita, Elsa
dc.date.accessioned2018-12-07T14:58:08Z
dc.date.available2018-12-07T14:58:08Z
dc.date.issued2017-10
dc.description.abstractCryopreservation of Senegalese sole sperm can represent an alternative to overcome some reproductive problems of this species. However, it is important to guarantee the safe use of cryopreserved sperm by selecting an appropriate protocol according to a high demand quality need to be ensured. It has been demonstrated that traditional assays such as motility and viability do not provide enough information to identify specific damage caused by cryopreservation process (freezing and thawing). Specific tests, including lipid peroxidation and DNA damage, should be performed. In the present study, motility and lipid peroxidation were performed as specific tests allowing us to discard cryopreservation conditions such as methanol as internal cryoprotectant and bovine serum albumin as external cryoprotectant. In addition, a caspase 3/7 detection by flow cytometry was performed to analyze apoptosis activity in the best selected conditions. Moreover, new highly sensitive tests based on transcript number detection have recently been described in fish sperm cryopreservation. For this reason, a transcript level detection assay was performed on certain oxidative and chaperone genes related to fertilization ability and embryo development (hsp70, hsp90BB, hsp90AA, gpx) to select the best cryopreservation conditions. DMSO+ egg yolk proved to be the best cryoprotectant combination in terms of transcript level. This study describes an optimized cryopreservation protocol for Solea senegalensis sperm demonstrating for the first time that transcript degradation is the most sensitive predictor of cell status in this species after cryopreservation.
dc.description.sponsorshipPROMAR Program [REPLING 31-03-05-FEP-69, KLING 31-03-05-FEP-73]; COST action (Food and Agriculture) AQUAGAMETE [FA1205]; Fundagao para a Ciencia e Tecnologia (FCT, Portugal) [CCMAR/Multi/04326/2013]; REPLING project [CCMAR/SC/BPD/09/2016]; FCT postdoctoral fellowship [SFRH/BPD/63933/2009]
dc.identifier.doi10.1371/journal.pone.0186542
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/10400.1/11872
dc.language.isoeng
dc.peerreviewedyes
dc.publisherPublic Library Science
dc.subjectTrout oncorhynchus mykiss
dc.subjectEarly embryo development
dc.subjectRainbow trout
dc.subjectDna damage
dc.subjectGene expression
dc.subjectGlutathione peroxidase-1
dc.subjectCyprinus carpio
dc.subjectMessenger rnas
dc.subjectCommon carp
dc.subjectL. Sperm
dc.titleSolea senegalensis sperm cryopreservation: new insights on sperm quality
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue10
oaire.citation.startPagee0186542
oaire.citation.titlePlos One
oaire.citation.volume12
person.familyNameF RIESCO
person.familyNameOliveira
person.familyNameSoares
person.familyNameGavaia
person.familyNameDinis
person.familyNameCabrita
person.givenNameMARTA
person.givenNameCatarina
person.givenNameFlorbela
person.givenNamePaulo
person.givenNameMaria Teresa
person.givenNameElsa
person.identifier.ciencia-id0816-30C5-51AC
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person.identifier.ciencia-idB619-FC16-D007
person.identifier.ciencia-id5414-4ACF-BF90
person.identifier.orcid0000-0002-3655-6401
person.identifier.orcid0000-0002-6679-761X
person.identifier.orcid0000-0001-6075-2472
person.identifier.orcid0000-0002-9582-1957
person.identifier.orcid0000-0001-7813-1324
person.identifier.orcid0000-0003-3864-8841
person.identifier.ridO-9766-2014
person.identifier.ridA-6470-2011
person.identifier.ridE-8523-2012
person.identifier.scopus-author-id54796976600
person.identifier.scopus-author-id35433190500
person.identifier.scopus-author-id35883720000
person.identifier.scopus-author-id6507104377
person.identifier.scopus-author-id7004150905
person.identifier.scopus-author-id26645697000
rcaap.rightsopenAccess
rcaap.typearticle
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