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Accelerated dynamic light sheet microscopy: unifying time-varying patterned illumination and low-rank and sparsity constrained reconstruction

2025-02-10Journal article Open access
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dc.contributor.authorVitali, Marco Tobia
dc.contributor.authorCandeo, Alessia
dc.contributor.authorFarina, Andrea
dc.contributor.authorPozzi, Paolo
dc.contributor.authorBrix, Alessia
dc.contributor.authorBassi, Andrea
dc.contributor.authorCorreia, Teresa
dc.date.accessioned2025-03-05T14:42:09Z
dc.date.available2025-03-05T14:42:09Z
dc.date.issued2025-02-10
dc.description.abstractLight Sheet Fluorescence Microscopy (LSFM) enables rapid and gentle 3D fluorescence imaging of dynamic processes over extended periods in translucent samples at the mesoscopic scale. However, its temporal resolution is constrained by the sequential acquisition of individual two-dimensional planes at varying depths, making it challenging to capture rapid dynamics such as the beating of a zebrafish heart. To address this limitation, we recently developed spatially modulated Selective Volume Illumination Microscopy, which utilizes a compressed sensing approach to reconstruct the entire imaging volume from measurements where multiple planes are illuminated simultaneously using spatially modulated light. Building on this advancement, we now introduce a novel spatio-temporal patterned illumination strategy and volume reconstruction method that incorporates low-rank and sparsity constraints, effectively leveraging the temporal and spatial redundancy present in sequential volumetric acquisitions. This method was applied to the volumetric imaging of embryonic zebrafish hearts, achieving an improvement in imaging speed of 4-fold compared to standard LSFM and a 2-fold improvement compared to traditional compressed sensing approaches, while preserving reconstruction accuracy and enabling the visualization of fast dynamic events with a resolution of a few tens of milliseconds. Our approach represents a step forward in enhancing the temporal resolution of LSFM for studying fast biological dynamics.eng
dc.description.sponsorshipLCF/PR/HR22/00533; 871124; 101094250; IR0000016; CUP B53C22001750006
dc.identifier.doi10.1088/2515-7647/adad23
dc.identifier.issn2515-7647
dc.identifier.urihttp://hdl.handle.net/10400.1/26855
dc.language.isoeng
dc.peerreviewedyes
dc.publisherIOP Publishing
dc.relationAlgarve Centre for Marine Sciences
dc.relationAlgarve Centre for Marine Sciences
dc.relationCentre for Marine and Environmental Research
dc.relationOPTical Imaging of Molecular and signalling Activity in Real-time: application to flatfish metamorphosis
dc.relation.ispartofJournal of Physics: Photonics
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectLight sheet
dc.subjectLow-rank
dc.subjectSparsity
dc.subjectDigital micro-mirror device
dc.subjectHadamard patterns
dc.titleAccelerated dynamic light sheet microscopy: unifying time-varying patterned illumination and low-rank and sparsity constrained reconstructioneng
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardTitleCentre for Marine and Environmental Research
oaire.awardTitleOPTical Imaging of Molecular and signalling Activity in Real-time: application to flatfish metamorphosis
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04326%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04326%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0101%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/EC/H2020/867450/EU
oaire.citation.issue2
oaire.citation.startPage025005
oaire.citation.titleJPhys: Photonics
oaire.citation.volume7
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamH2020
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameCorreia
person.givenNameTeresa
person.identifier.ciencia-idF01E-082A-5B36
person.identifier.orcid0000-0002-1606-9550
person.identifier.scopus-author-id23392190500
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameEuropean Commission
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relation.isAuthorOfPublication.latestForDiscovery2217fb83-cb80-4f7b-b039-6476ddb910a5
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