Repository logo
 
Publication

Successful cryopreservation of sperm from sex-reversed dusky grouper, Epinephelus marginatus

2009Journal article Restricted access
Simple item page
dc.contributor.authorCabrita, Elsa
dc.contributor.authorEngrola, S.
dc.contributor.authorConceição, L. E. C.
dc.contributor.authorPousão-Ferreira, P.
dc.contributor.authorDinis, Maria Teresa
dc.date.accessioned2014-07-03T14:40:06Z
dc.date.available2014-07-03T14:40:06Z
dc.date.issued2009
dc.date.updated2014-07-03T11:18:25Z
dc.description.abstractThe dusky grouper, Epinephelus marginatus, is a protogynous monandric hermaphrodite species, since individuals mature firstly as females and later as males. This makes capture and maintenance of males in captivity difficult due to their large size and older age. Thus, the use of techniques that allow controlling sex, obtaining smaller and younger males, as well as cryopreservation techniques to improve sperm availability, may contribute to improve dusky grouper reproduction in captivity. In the present study, immature fish and young females were sex reversed using 2.5 mg/kg BW 17α-methyltestosterone (α-MT) in silastic implants, two months prior gamete extraction. In all individuals a single dose of Lucrin Depot® (20 μg/kg BW, GnRHa) was enough to induce and/or increase sperm production. Volume of sperm, cell concentration, osmolarity, sperm production and motility were registered. Sperm was cryopreserved in 0.5 ml straws using 1% NaCl + 10% DMSO + 10 mg/ml BSA as extender. Post-thawed sperm quality was analysed in terms of motility, viability and fertilization. The sperm volume collected ranged from 5 to 400 μl, cell density from 1.2 to 16.3 × 109 spz/ml and sperm production from 0.04 to 3.9 × 109 spermatozoa. The percentage of motile cells at 15 s post-activation varied from 25% to 93%. Cell viability decreased in post-thawed samples (22.5%) as well as the percentage of motile cells (36.8%). However, sperm velocity (VCL and VSL) and movement pattern (Lin) were not significantly affected by cryopreservation and spermatozoa were able to fertilize the oocytes without a decrease in the fertilization rate. Fertilization rates ranged in thawed samples from 35.9% to 65.1% and only one sample was significantly different from the control (69.5%). Embryo development was impaired in some fertilization trails, registering a significant decrease in the percentage of embryos at G stage when compared with the control. This suggests that the quality of samples may be the principal requirement for improving fertilization rather than the optimization of the cryopreservation protocol. The present study demonstrated that the protocol used for sperm cryopreservation can be successfully used in sex-reversed males for the establishment of a sperm bank. This technique would contribute to the reproduction of this species benefiting production, sea-ranching and species conservation.por
dc.identifier.citationCabrita, E.; Engrola, S.; Conceição, L.E.C.; Pousão-Ferreira, P.; Dinis, M.T.Successful cryopreservation of sperm from sex-reversed dusky grouper, Epinephelus marginatus, Aquaculture, 287, 1-2, 152-157, 2009.por
dc.identifier.doihttp://dx.doi.org/ 10.1016/j.aquaculture.2008.10.019
dc.identifier.issn0044-8486
dc.identifier.urihttp://hdl.handle.net/10400.1/4642
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S0044848608007503por
dc.subjectSperm cryopreservationpor
dc.subjectSex-reversalpor
dc.subjectGrouperpor
dc.subjectSperm qualitypor
dc.subjectEpinephelus marginatuspor
dc.titleSuccessful cryopreservation of sperm from sex-reversed dusky grouper, Epinephelus marginatuspor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage157por
oaire.citation.issue1-2por
oaire.citation.startPage152por
oaire.citation.titleAquaculturepor
oaire.citation.volume287por
person.familyNameCabrita
person.familyNameEngrola
person.familyNamePousão-Ferreira
person.familyNameDinis
person.givenNameElsa
person.givenNameSofia
person.givenNamePedro
person.givenNameMaria Teresa
person.identifier237846
person.identifier.ciencia-idA610-29E7-9B83
person.identifier.ciencia-id5A11-AB29-0849
person.identifier.ciencia-id5414-4ACF-BF90
person.identifier.orcid0000-0003-3864-8841
person.identifier.orcid0000-0002-5244-5541
person.identifier.orcid0000-0001-6746-764X
person.identifier.orcid0000-0001-7813-1324
person.identifier.ridE-8523-2012
person.identifier.ridA-2485-2012
person.identifier.ridH-3689-2014
person.identifier.scopus-author-id26645697000
person.identifier.scopus-author-id8669620600
person.identifier.scopus-author-id6506349877
person.identifier.scopus-author-id7004150905
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication332fa9b9-09b5-47fe-a1b3-afe088b4150a
relation.isAuthorOfPublication51be5a20-df9c-4a90-85eb-51d779642caa
relation.isAuthorOfPublicationf50ae82d-d5c6-49e4-8562-e45dc502e7d4
relation.isAuthorOfPublication309dec19-8aa9-4bea-8665-42dd4b3a9894
relation.isAuthorOfPublication.latestForDiscovery51be5a20-df9c-4a90-85eb-51d779642caa

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Successful cryopreservation of sperm from sex-reversed dusky grouper, Epinephelus marginatus.pdf
Size:
512.73 KB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.61 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

CCM2-Artigos (em revistas ou actas indexadas)