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Physical mapping in a triplicated genome: mapping the downy mildew resistance locus Pp523 in Brassica oleracea L.

2011Journal article Open access
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dc.contributor.authorCarlier, Jorge
dc.contributor.authorAlabaça, Cláudia S.
dc.contributor.authorSousa, N. H.
dc.contributor.authorCoelho, P. S.
dc.contributor.authorMonteiro, A. A.
dc.contributor.authorPaterson, A. H.
dc.contributor.authorLeitão, J. M.
dc.date.accessioned2015-06-19T14:36:25Z
dc.date.available2015-06-19T14:36:25Z
dc.date.issued2011
dc.description.abstractWe describe the construction of a BAC contig and identification of a minimal tiling path that encompass the dominant and monogenically inherited downy mildew resistance locus Pp523 of Brassica oleracea L. The selection of BAC clones for construction of the physical map was carried out by screening gridded BAC libraries with DNA overgo probes derived from both genetically mapped DNA markers flanking the locus of interest and BAC-end sequences that align to Arabidopsis thaliana sequences within the previously identified syntenic region. The selected BAC clones consistently mapped to three different genomic regions of B. oleracea. Although 83 BAC clones were accurately mapped within a similar to 4.6 cM region surrounding the downy mildew resistance locus Pp523, a subset of 33 BAC clones mapped to another region on chromosome C8 that was similar to 60 cM away from the resistance gene, and a subset of 63 BAC clones mapped to chromosome C5. These results reflect the triplication of the Brassica genomes since their divergence from a common ancestor shared with A. thaliana, and they are consonant with recent analyses of the C genome of Brassica napus. The assembly of a minimal tiling path constituted by 13 (BoT01) BAC clones that span the Pp523 locus sets the stage for map-based cloning of this resistance gene.
dc.identifier.doihttps://dx.doi.org/10.1534/g3.111.001099
dc.identifier.issn2160-1836
dc.identifier.otherAUT: JLE00446;
dc.identifier.urihttp://hdl.handle.net/10400.1/6492
dc.language.isoeng
dc.peerreviewedyes
dc.publisherGenetics Society of America
dc.relationSFRH/BDP/41714/2007
dc.relationCloning Pp523, a downy mildew resistance gene of Brassica oleracea L.
dc.relation.isbasedonP-002-JCV
dc.titlePhysical mapping in a triplicated genome: mapping the downy mildew resistance locus Pp523 in Brassica oleracea L.
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleCloning Pp523, a downy mildew resistance gene of Brassica oleracea L.
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FAGR-GPL%2F70135%2F2006/PT
oaire.citation.endPage601
oaire.citation.startPage593
oaire.citation.titleG3 - Genes Genomes Genetics
oaire.citation.volume1
oaire.fundingStream3599-PPCDT
person.familyNameCarlier
person.familyNameLeitão
person.givenNameJorge
person.givenNameJosé
person.identifier258398
person.identifier.ciencia-id691D-A1BB-15CC
person.identifier.ciencia-id7A17-3E0A-73AD
person.identifier.orcid0000-0003-0675-2716
person.identifier.orcid0000-0003-4278-0415
person.identifier.ridAAH-3958-2020
person.identifier.scopus-author-id7102166949
person.identifier.scopus-author-id7005119796
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccess
rcaap.typearticle
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