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Enhancing efficiency of enzymatic-assisted extraction method for evaluating bioactive compound analysis in mulberry: an optimization approach

2023-10Journal article Open access
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dc.contributor.authorTizón Alba, Ainara
dc.contributor.authorAliaño-González, M.J.
dc.contributor.authorPalma, Miguel
dc.contributor.authorFernández Barbero, Gerardo
dc.contributor.authorCarrera, Ceferino
dc.date.accessioned2023-11-27T16:21:01Z
dc.date.available2023-11-27T16:21:01Z
dc.date.issued2023-10
dc.description.abstractThe present investigation endeavors to optimize a method based on enzyme-assisted extraction for the efficient retrieval of bioactive compounds from mulberry, leveraging its notable health-promoting properties. A combined approach of Plackett-Burman design followed by Box-Behnken design was employed for determining the crucial extraction parameters and subsequently, refining the process. Optimal conditions consisted of heating 0.15 g of mulberry at 40 degrees C, using 15 mL of 70% EtOH as a solvent at pH 4, 38.46 enzyme units per g of sample, and shaking at 200 rpm. The optimum extraction time study revealed that 5 min of extraction was sufficient to reach the maximum concentration of the bioactive compound. The repeatability and intermediate precision assessment exhibited a coefficient of variation below 5%. Among the diverse mulberry varieties scrutinized, Morus nigra showed the highest anthocyanin content (27.90 +/- 2.14 mg/100 g), while Morus rubra showed the highest concentration of phenolic compounds (121.10 +/- 19.56 mg/100 g). Moreover, the extracted compounds showcased significant antioxidant and antimicrobial properties.pt_PT
dc.description.sponsorshipEquipment for liquid chromatography by means of mass spectrometry and ion chromatography) of the State Subprogram of Research Infrastructures and Technical Scientific Equipment EQC2018-005135-Ppt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.3390/agronomy13102548pt_PT
dc.identifier.eissn2073-4395
dc.identifier.urihttp://hdl.handle.net/10400.1/20170
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectMulberriespt_PT
dc.subjectEnzyme-assisted extractionpt_PT
dc.subjectAntioxidant activitypt_PT
dc.subjectAntimicrobial propertiespt_PT
dc.subjectBox-Behnken optimizationpt_PT
dc.subjectPlackett-BurmanEQC2018-005135-Ppt_PT
dc.titleEnhancing efficiency of enzymatic-assisted extraction method for evaluating bioactive compound analysis in mulberry: an optimization approachpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue10pt_PT
oaire.citation.startPage2548pt_PT
oaire.citation.titleAgronomypt_PT
oaire.citation.volume13pt_PT
person.familyNameAliaño González
person.givenNameMaría José
person.identifier456042
person.identifier.ciencia-idDD17-3640-DB88
person.identifier.orcid0000-0002-5816-4476
person.identifier.scopus-author-id57200221253
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication305fc84f-d79e-49d4-ba14-bea107dcc203
relation.isAuthorOfPublication.latestForDiscovery305fc84f-d79e-49d4-ba14-bea107dcc203

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