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Changes in the soluble bone proteome of reared white seabream (Diplodus sargus) with skeletal deformities

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dc.contributor.authorSilva, Tomé S.
dc.contributor.authorCordeiro, O.
dc.contributor.authorRichard, Nadège
dc.contributor.authorConceição, L. E. C.
dc.contributor.authorRodrigues, Pedro
dc.date.accessioned2014-06-18T10:22:30Z
dc.date.available2014-06-18T10:22:30Z
dc.date.issued2011
dc.date.updated2014-06-12T11:33:36Z
dc.description.abstractOne of the main constrains for commercial aquaculture production of white seabream (Diplodus sargus) is the high incidence of skeletal malformations in reared fish. The purpose of this study was to obtain a better understanding of the mechanisms involved in the development of these types of skeletal malformations by comparative proteomic analysis of the vertebral column of normal and deformed fish using 2DE for protein separation and MS for protein identification. We observed a 3.2 and 3.4-fold increase in the expression of two tropomyosin isoforms, one of which (tropomyosin-4) is essential for the motility and polarization cycles of osteoclasts. Furthermore, a 1.6, 1.7 and 1.8-fold increase in three parvalbumin spots was detected, suggesting a cellular response to increased intracellular Ca2+ levels. These results can be interpreted as signs of increased cellular activity in the bone of white seabream with skeletal deformities coupled to a higher degree of calcium mobilization, which elicits further studies into the use of these proteins as indicators of skeletal metabolic state.por
dc.identifier.citationSilva, Tomé S.; Cordeiro, Odete; Richard, Nadège; Conceição, Luís E.C.; Rodrigues, Pedro M. Changes in the soluble bone proteome of reared white seabream (Diplodus sargus) with skeletal deformities, Comparative Biochemistry and Physiology Part D: Genomics and Proteomics, 6, 1, 82-91, 2011.por
dc.identifier.doihttp://dx.doi.org/10.1016/j.cbd.2010.03.008
dc.identifier.issn1744-117X
dc.identifier.otherAUT: PMR01268;
dc.identifier.urihttp://hdl.handle.net/10400.1/4365
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.subjectAquaculturepor
dc.subjectChemically assisted fragmentationpor
dc.subjectDifferential expressionpor
dc.subjectMASCOTpor
dc.subjectSkeletal deformitiespor
dc.titleChanges in the soluble bone proteome of reared white seabream (Diplodus sargus) with skeletal deformitiespor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage91por
oaire.citation.issue1por
oaire.citation.startPage82por
oaire.citation.titleComparative Biochemistry and Physiology Part D: Genomics and Proteomicspor
oaire.citation.volume6por
person.familyNameRichard
person.familyNameRodrigues
person.givenNameNadège
person.givenNamePedro
person.identifier.orcid0000-0002-8983-0726
person.identifier.orcid0000-0002-9668-1204
person.identifier.ridM-3385-2013
person.identifier.ridM-3406-2013
person.identifier.scopus-author-id15023061500
person.identifier.scopus-author-id55107531000
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublicationf372969b-5422-4111-96cd-447a96f7e4f4
relation.isAuthorOfPublicationc4613fe6-e1a0-4dd9-be8d-442b2b8a7b72
relation.isAuthorOfPublication.latestForDiscoveryf372969b-5422-4111-96cd-447a96f7e4f4

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