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Fluid flow and interlinked feedback loops establish left-right asymmetric decay of Cerl2 mRNA

2012-12Journal article Open access
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dc.contributor.authorNakamura, Tetsuya
dc.contributor.authorSaito, Daisuke
dc.contributor.authorKawasumi, Aiko
dc.contributor.authorShinohara, Kyosuke
dc.contributor.authorAsai, Yasuko
dc.contributor.authorTakaoka, Katsuyoshi
dc.contributor.authorDong, Fenglan
dc.contributor.authorTakamatsu, Atsuko
dc.contributor.authorA. Belo, José
dc.contributor.authorMochizuki, Atsushi
dc.contributor.authorHamada, Hiroshi
dc.date.accessioned2018-12-07T14:52:49Z
dc.date.available2018-12-07T14:52:49Z
dc.date.issued2012-12
dc.description.abstractBreaking of left-right symmetry in mouse embryos requires fluid flow at the node, but the precise action of the flow has remained unknown. Here we show that the left-right asymmetry of Cerl2 expression around the node, a target of the flow, is determined post-transcriptionally by decay of Cerl2 mRNA in a manner dependent on its 3' untranslated region. Cerl2 mRNA is absent specifically from the apical region of crown cells on the left side of the node. Preferential decay of Cerl2 mRNA on the left is initiated by the leftward flow and further enhanced by the operation of Wnt-Cerl2 interlinked feedback loops, in which Wnt3 upregulates Wnt3 expression and promotes Cerl2 mRNA decay, whereas Cerl2 promotes Wnt degradation. Mathematical modelling and experimental data suggest that these feedback loops behave as a bistable switch that can amplify in a noise-resistant manner a small bias conferred by fluid flow.
dc.description.sponsorshipMinistry of Education, Culture, Sports, Science, and Technology of Japan; Core Research for Evolutional Science and Technology (CREST) of the Japan Science and Technology Corporation (JST); GCOE of Osaka University; FCT
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.doi10.1038/ncomms2319
dc.identifier.issn2041-1723
dc.identifier.urihttp://hdl.handle.net/10400.1/11225
dc.language.isoeng
dc.peerreviewedyes
dc.publisherNature Publishing Group
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectLeft right axis
dc.subjectMouse embryo
dc.subjectGene expression
dc.subjectAnteroposterior Axis
dc.subjectLateral plate
dc.subjectBeta
dc.subjectSystem
dc.subjectNode
dc.subjectCell
dc.subjectSegmentation
dc.titleFluid flow and interlinked feedback loops establish left-right asymmetric decay of Cerl2 mRNA
dc.typejournal article
dspace.entity.typePublication
oaire.citation.startPage1322
oaire.citation.titleNature Communications
oaire.citation.volume3
person.familyNameBelo
person.givenNameJosé A.
person.identifier.ciencia-idBF13-08E9-25E6
person.identifier.orcid0000-0001-7384-0949
person.identifier.ridF-4444-2012
person.identifier.scopus-author-id6602141392
rcaap.rightsopenAccess
rcaap.typearticle
relation.isAuthorOfPublication4bebeb48-6fac-41a9-8363-f849def5e019
relation.isAuthorOfPublication.latestForDiscovery4bebeb48-6fac-41a9-8363-f849def5e019

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