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Drospirenone intake alters plasmatic steroid levels and cyp17a1 expression in gonads of juvenile sea bass

2016-07Journal article Open access
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dc.contributor.authorBlanco, Maria
dc.contributor.authorFernandes, Denise
dc.contributor.authorMedina, Paula
dc.contributor.authorBlazquez, Mercedes
dc.contributor.authorPorte, Cinta
dc.date.accessioned2017-04-07T15:56:35Z
dc.date.available2017-04-07T15:56:35Z
dc.date.issued2016-07
dc.description.abstractDrospirenone (DRO) is one of the most widely used progestins in contraceptive treatments and hormone replacement therapies. The pharmacokinetics and potential toxicological effects of DRO were investigated in juvenile sea bass (Dicentrarchus labrax) exposed through the diet (0.01-10 mu g DRO/g) for up to 31 days. DRO was detected in the blood (4-27 ng/mL) of fish exposed to the highest concentration, with no significant bioaccumulation over time and no alteration of hepatic metabolizing enzymes, namely, CYP1A and CYP3A-catalysed activities and UDP-glucuronyltransferase (UGT). Pregnenolone (P5), progesterone (P4), 17 alpha-hydroxyprogesterone (17P4), 17 alpha-hydroxypregnenolone (17P5), androstenedione (AD) and testosterone (T) were determined in plasma and gene expression of cyp17a1, cyp19a1a and cyp11 beta analysed by qRT-PCR in gonads. The significant increase in plasmatic levels of 17P5, 17P4 and AD detected after 31 days exposure to 10 ng DRO/g together with the increased expression of cyp17a1 in females evidence the ability of DRO to alter steroid synthesis at low intake concentrations (7 ng DRO/day). However, the potential consequences of this steroid shift for female reproduction remain to be investigated. (C) 2016 Elsevier Ltd. All rights reserved.
dc.description.sponsorshipSpanish Government and Paula Medina the pre-doctoral fellowship MECE2, ANT0806; Spanish National Plan of Research (Project Ref. CGL2011-24538)
dc.identifier.doi10.1016/j.envpol.2016.03.007
dc.identifier.issn0269-7491
dc.identifier.urihttp://hdl.handle.net/10400.1/9462
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier
dc.relationPPARS/RXR RECEPTORS: NEW TOOLS FOR THE DETECTION OF THE EFFECTS OF PHARMACEUTICALS AND BIOLOGICALLY ACTIVE COMPOUNDS IN THE AQUATIC ENVIRONMENT
dc.relation.isbasedonWOS:000377921800057
dc.subjectProgestins
dc.subjectSea bass
dc.subjectSex steroids
dc.subjectMetabolism
dc.subjectcyp17a1
dc.titleDrospirenone intake alters plasmatic steroid levels and cyp17a1 expression in gonads of juvenile sea bass
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitlePPARS/RXR RECEPTORS: NEW TOOLS FOR THE DETECTION OF THE EFFECTS OF PHARMACEUTICALS AND BIOLOGICALLY ACTIVE COMPOUNDS IN THE AQUATIC ENVIRONMENT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F34289%2F2006/PT
oaire.citation.endPage548
oaire.citation.startPage541
oaire.citation.titleEnvironmental Pollution
oaire.citation.volume213
person.familyNameFernandes
person.givenNameDenise
person.identifier.orcid0000-0003-0599-3200
person.identifier.scopus-author-id7005622274
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccess
rcaap.typearticle
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relation.isAuthorOfPublication.latestForDiscovery10f0a734-62a4-4bdd-af9b-afacef0e8a2e
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