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Spectroscopic characterization of a novel 2×[4Fe–4S] ferredoxin isolated from Desulfovibrio desulfuricans ATCC 27774

2003Journal article Restricted access
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dc.contributor.authorRodrigues, Pedro
dc.contributor.authorMoura, Isabel
dc.contributor.authorMacedo, Anjos L.
dc.contributor.authorMoura, José J. G.
dc.date.accessioned2014-06-19T09:29:55Z
dc.date.available2014-06-19T09:29:55Z
dc.date.issued2003
dc.date.updated2014-06-12T13:42:00Z
dc.description.abstractA novel iron /sulfur containing protein, a ferredoxin (Fd), was purified to homogeneity from the extract of Desulfovibrio desulfuricans American type culture collection (ATCC) 27774. The purified protein is a 13.4 kDa homodimer with a polypeptide chain of 60 amino acids residues, containing eight cysteines that coordinate two [4Fe /4S] clusters. The protein is shown to be air sensitive and cluster conversions take place. We structurally characterize a redox state that contains two [4Fe /4S] cores. 1D and 2D 1H NMR studies are reported on form containing the clusters in the oxidized state. Based on the nuclear Overhauser effect (NOE), relaxation measurements and comparison of the present data with the available spectra of the analogous 8Fe Fds, the cluster ligands were specifically assigned to the eight-cysteinyl residues.por
dc.identifier.citationRodrigues, Pedro M; Moura, Isabel; Macedo, Anjos L; Moura, José J.G. Spectroscopic characterization of a novel 2×[4Fe–4S] ferredoxin isolated from Desulfovibrio desulfuricans ATCC 27774, Inorganica Chimica Acta, 356, 1, 215-221, 2003.por
dc.identifier.doihttp://dx.doi.org/10.1016/S0020-1693(03)00472-9
dc.identifier.issn0020-1693
dc.identifier.otherAUT: PMR01268;
dc.identifier.urihttp://hdl.handle.net/10400.1/4405
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.subjectIron-sulfur clusterpor
dc.subject2x/[4Fe-/4S] clusterspor
dc.subjectFerredoxinpor
dc.subjectMetalloproteinpor
dc.subjectParamagnetic proteinpor
dc.subjectNuclear magnetic resonancepor
dc.subjectDesulfovibrio desulfuricans ATCC 27774por
dc.titleSpectroscopic characterization of a novel 2×[4Fe–4S] ferredoxin isolated from Desulfovibrio desulfuricans ATCC 27774por
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage221por
oaire.citation.issue1por
oaire.citation.startPage215por
oaire.citation.titleInorganica Chimica Actapor
oaire.citation.volume356por
person.familyNameRodrigues
person.givenNamePedro
person.identifier.orcid0000-0002-9668-1204
person.identifier.ridM-3406-2013
person.identifier.scopus-author-id55107531000
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublicationc4613fe6-e1a0-4dd9-be8d-442b2b8a7b72
relation.isAuthorOfPublication.latestForDiscoveryc4613fe6-e1a0-4dd9-be8d-442b2b8a7b72

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