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'Smelling' the cerebrospinal fluid: olfactory signaling molecules are expressed in and mediate chemosensory signaling from the choroid plexus

2016-05Artigo científico Acesso restrito
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dc.contributor.authorGonçalves, Isabel
dc.contributor.authorHubbard, Peter
dc.contributor.authorTomás, Joana
dc.contributor.authorQuintela, Telma
dc.contributor.authorTavares, Gabriela
dc.contributor.authorCaria, Sandra
dc.contributor.authorBarreiros, Daniela
dc.contributor.authorSantos, Cecilia
dc.date.accessioned2017-04-07T15:56:42Z
dc.date.available2017-04-07T15:56:42Z
dc.date.issued2016-05
dc.description.abstractThe olfactory-type signaling machinery has been known to be involved not only in odorant detection but also in other tissues with unsuspected sensory roles. As a barrier, the choroid plexus (CP) is an active participant in the monitoring of the cerebrospinal fluid (CSF), promptly responding to alterations in its composition. We hypothesized that olfactory signaling could be active in CP, contributing to the surveillance of the CSF composition. We determined the mRNA and protein expression of the major components of the olfactory transduction pathway in the rat CP, including odorant receptors, the olfactory G-protein (Gaolf), adenylate cyclase 3 and cyclic nucleotide-gated channel 2. The functionality of the transduction pathway and the intracellular mechanisms involved were analyzed by DC field potential recording electrophysiological analysis, in an ex vivo CP-brain setup, using polyamines as stimuli and blockers of the downstream signaling pathways. Concentration-dependent responses were obtained for the polyamines studied (cadaverine, putrescine, spermine and spermidine), all known to be present in the CSF. Transfection of a CP epithelial cell line with siRNA against Gaolf effectively knocked down protein expression and reduced the CP cells' response to spermine. Thus, the key components of the olfactory chemosensory apparatus are present and are functional in murine CP, and polyamines seem to trigger both the cAMP and the phospholipase C-inositol 1,4,5-trisphosphate pathways. Olfactory-like chemosensory signaling may be an essential component of the CP chemical surveillance apparatus to detect alterations in the CSF composition, and to elicit responses to modulate and maintain brain homeostasis.
dc.identifier.doi10.1111/febs.13700
dc.identifier.issn1742-464X
dc.identifier.urihttp://hdl.handle.net/10400.1/9499
dc.language.isoeng
dc.peerreviewedyes
dc.publisherWiley
dc.relationMicroarray based identification of estrogen and androgen responsive genes in rat choroid plexus
dc.relationMICROARRAY BASED IDENTIFICATION OF ESTROGEN RESPONSIVE GENES IN RAT CHOROID PLEXUS
dc.relation.isbasedonWOS:000376601900012
dc.subjectCerebrospinal fluid
dc.subjectChoroid plexus
dc.subjectOdorant receptors
dc.subjectOlfactory
dc.subjectPolyamines
dc.title'Smelling' the cerebrospinal fluid: olfactory signaling molecules are expressed in and mediate chemosensory signaling from the choroid plexus
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleMicroarray based identification of estrogen and androgen responsive genes in rat choroid plexus
oaire.awardTitleMICROARRAY BASED IDENTIFICATION OF ESTROGEN RESPONSIVE GENES IN RAT CHOROID PLEXUS
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876-PPCDTI/PTDC%2FSAU-NMC%2F114800%2F2009/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F00709%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F70781%2F2010/PT
oaire.citation.endPage1766
oaire.citation.issue9
oaire.citation.startPage1748
oaire.citation.titleFEBS Journal
oaire.citation.volume283
oaire.fundingStream5876-PPCDTI
oaire.fundingStream5876
person.familyNameHubbard
person.familyNameSantos
person.givenNamePeter
person.givenNameCecilia
person.identifier.ciencia-id951F-BA4B-A21F
person.identifier.ciencia-id761A-7ED2-D68F
person.identifier.orcid0000-0002-3007-4647
person.identifier.orcid0000-0001-6074-7825
person.identifier.ridB-2823-2008
person.identifier.ridC-9032-2016
person.identifier.scopus-author-id7005928467
person.identifier.scopus-author-id7201458751
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccess
rcaap.typearticle
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relation.isAuthorOfPublication0f8d7c69-8b13-4668-a14b-2d4d6741a6dd
relation.isAuthorOfPublication.latestForDiscovery0f8d7c69-8b13-4668-a14b-2d4d6741a6dd
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