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Polymorphic microsatellite markers in the brown seaweed Fucus vesiculosus

2015-03-08Journal article Open access
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dc.contributor.authorCandeias, Rui
dc.contributor.authorCasado-Amezúa, Pilar
dc.contributor.authorPearson, G. A.
dc.contributor.authorSerrão, Ester
dc.contributor.authorTeixeira, Sara
dc.date.accessioned2015-09-08T10:57:43Z
dc.date.available2015-09-08T10:57:43Z
dc.date.issued2015-03-08
dc.date.updated2015-08-03T15:57:10Z
dc.description.abstractBackground: Fucus vesiculosus is a brown seaweed dominant on temperate rocky shores of the northern hemisphere and, is typically distributed in the mid-upper intertidal zone. It is an external fertilizer that reproduces sexually, providing an excellent model to address conflicting theories related to mating systems and sexual selection. Microsatellite markers have been reported for several Fucus species, however the genomic libraries from where these markers have been isolated, have originated from two or more species pooled together (F. vesiculosus and F. serratus in one library; F. vesiculosus, F. serratus and Ascophyllum nodosum in a second library), or when the genomic DNA originated from only one species it was from Fucus spiralis. Although these markers cross-amplify F. vesiculosus individuals, the level of polymorphism has been low for relatedness studies. Findings: The microsatellite markers described here were obtained from an enriched genomic library, followed by 454 pyrosequencing. A total of 9 microsatellite markers were tested across 44 individuals from the North of Portugal. The mean number of alleles across loci was 8.7 and the gene diversity 0.67. Conclusions: The high variability displayed by these microsatellite loci should be useful for paternity analysis, assessing variance of reproductive success and in estimations of genetic variation within and between populations.por
dc.description.sponsorshipFundação para a Ciência e Tecnologiapor
dc.description.sponsorshipEXCL/AAG-GLO/0661/2012
dc.identifier.citationBMC Research Notes. 2015 Mar 08;8(1):73por
dc.identifier.doi10.1186/s13104-015-1035-x
dc.identifier.urihttp://hdl.handle.net/10400.1/6741
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherBiomed Centralpor
dc.relationPolyandry levels in Fucus vesiculosus
dc.rights.holderCandeias et al.; licensee BioMed Central.
dc.subjectIntertidalpor
dc.subjectMicrosatellitespor
dc.subjectGenetic diversitypor
dc.subjectFucus vesiculosuspor
dc.subjectBrown macroalgapor
dc.titlePolymorphic microsatellite markers in the brown seaweed Fucus vesiculosuspor
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitlePolyandry levels in Fucus vesiculosus
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FMAR%2F104477%2F2008/PT
oaire.citation.issue(1):73por
oaire.citation.titleBMC Research Notespor
oaire.citation.volume8por
oaire.fundingStream3599-PPCDT
person.familyNamePearson
person.familyNameSerrao
person.familyNameTeixeira
person.givenNameGareth Anthony
person.givenNameEster A.
person.givenNameSara
person.identifier113536
person.identifierC-6686-2012
person.identifier.ciencia-id3315-9919-1A52
person.identifier.ciencia-id5B13-B26E-B1EC
person.identifier.orcid0000-0002-0768-464X
person.identifier.orcid0000-0003-1316-658X
person.identifier.orcid0000-0003-0419-2348
person.identifier.ridJ-3911-2013
person.identifier.ridM-3424-2013
person.identifier.scopus-author-id55916875600
person.identifier.scopus-author-id7004093604
person.identifier.scopus-author-id23986333100
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication0a10c448-c7f5-4c3b-9488-3917c707e35e
relation.isAuthorOfPublication45ccfe90-155c-4d6f-9e86-8f0fd064005f
relation.isAuthorOfPublicationb461f1bb-dfdb-410f-b579-647fa97957ca
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