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Elicitation improves rosmarinic acid content and antioxidant activity in Thymus lotocephalus shoot cultures

2019-11Journal article Restricted access
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dc.contributor.authorGonçalves, Sandra
dc.contributor.authorMansinhos, Inês
dc.contributor.authorRodríguez Solana, Raquel
dc.contributor.authorPerez-Santin, Efren
dc.contributor.authorCoelho, Natacha
dc.contributor.authorRomano, Anabela
dc.date.accessioned2020-07-24T10:51:35Z
dc.date.available2020-07-24T10:51:35Z
dc.date.issued2019-11
dc.description.abstractThymus lotocephalus G. Lopez & R. Morales is an aromatic species endemic to the south of Portugal with medicinal properties. The aim of this work was to evaluate the effect of elicitors, cytokinin, and different sucrose concentrations on chemical composition and antioxidant activity of extracts from in vitro regenerated shoots. Elicitors (YE: yeast extract, salicylic acid, and AgNO3) were added directly to the medium. Phytochemical analysis include evaluation of total phenolic (TPC), flavonoid (TFC), and rosmarinic acid (RAC) contents. The TPCs and TFCs were determined by Folin-Ciocalteu and aluminum chloride calorimetric assays, respectively. Rosmarinic acid (RA), the main compound found in the studied extracts, was quantified by liquid chromatography-photodiode array detector. Results showed that the cytokinin benzyladenine has an inhibitory effect on TPC, TFC and RAC, as well as on antioxidant activity. TPC was only significantly improved by the presence of YE (from 95.49 +/- 3.34 to 118.34 +/- 6.24 mg(GAE)/g(extract)) and TFCs were not affected by elicitation. The increase in sucrose concentration, from 2 to 4%, and the three elicitors tested, particularly YE, enhanced RACs (from 48.61 +/- 0.09 in the control medium to 78.57 +/- 0.99 mg/g(extract) in YE-elicited medium). In accordance with these results, the antioxidant activity measured by 2,2-diphenyl-1-picrylhydrazyl, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), and ferric reducing antioxidant power assays was significantly greater in extracts from elicited shoots. Overall, the results obtained suggest that shoots of T. lotocephalus are a good source of antioxidant compounds and showed that the production of RA can be promoted by altering in vitro culture conditions.
dc.description.sponsorshipproject INTERREG - MD.Net: When Brand Meets People
dc.description.sponsorshipFoundation for Science and Technology (FCT), PortugalPortuguese Foundation for Science and Technology [SFRH/BPD/103086/2014]
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.doi10.1016/j.indcrop.2019.04.071
dc.identifier.issn0926-6690
dc.identifier.urihttp://hdl.handle.net/10400.1/14245
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier
dc.subjectIn-vitro culture
dc.subjectEssential oil
dc.subjectSecondary metabolism
dc.subjectSalicylic-acid
dc.subjectSuspension-cultures
dc.subjectPhenolic-compounds
dc.subjectExtracts
dc.subjectAccumulation
dc.subjectGrowth
dc.subjectMultiplication
dc.subjectCytokinin
dc.subjectRosmarinic acid
dc.subjectSalicylic acid
dc.subjectSilver nitrate
dc.subjectSucrose
dc.subjectYeast extract
dc.titleElicitation improves rosmarinic acid content and antioxidant activity in Thymus lotocephalus shoot cultures
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage220
oaire.citation.startPage214
oaire.citation.titleIndustrial Crops and Products
oaire.citation.volume137
person.familyNameGonçalves
person.familyNamePaixão Mansinhos
person.familyNameRodríguez Solana
person.familyNameCoelho
person.familyNameRomano
person.givenNameSandra
person.givenNameInês Filipa
person.givenNameRaquel
person.givenNameNatacha
person.givenNameAnabela
person.identifier624004
person.identifier.ciencia-id9F19-E3AE-2C59
person.identifier.ciencia-id4114-8891-54E1
person.identifier.ciencia-idFE10-7889-0562
person.identifier.ciencia-idC518-BDB3-E127
person.identifier.ciencia-id8A18-7E9A-3B0E
person.identifier.orcid0000-0002-3038-4434
person.identifier.orcid0000-0001-9632-8876
person.identifier.orcid0000-0003-4204-7387
person.identifier.orcid0000-0003-4265-5622
person.identifier.orcid0000-0002-7204-7428
person.identifier.ridM-6988-2017
person.identifier.ridI-6317-2015
person.identifier.scopus-author-id8583003200
person.identifier.scopus-author-id55081917100
person.identifier.scopus-author-id56249997600
rcaap.rightsrestrictedAccess
rcaap.typearticle
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relation.isAuthorOfPublicationc998debf-3780-44a4-a933-c7dc238dfe30
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