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Macroalgal morphogenesis induced by waterborne compounds and bacteria in coastal seawater

2016-01Journal article Open access
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dc.contributor.authorGrueneberg, Jan
dc.contributor.authorEngelen, Aschwin H.
dc.contributor.authorCosta, Rodrigo
dc.contributor.authorWichard, Thomas
dc.date.accessioned2017-04-07T15:57:17Z
dc.date.available2017-04-07T15:57:17Z
dc.date.issued2016-01
dc.description.abstractAxenic gametes of the marine green macroalga Ulva mutabilis Foyn (Ria Formosa, locus typicus) exhibit abnormal development into slow-growing callus-like colonies with aberrant cell walls. Under laboratory conditions, it was previously demonstrated that all defects in growth and thallus development can be completely abolished when axenic gametes are inoculated with a combination of two specific bacterial strains originally identified as Roseo-bacter sp. strain MS2 and Cytophaga sp. strain MS6. These bacteria release diffusible morphogenetic compounds (= morphogens), which act similar to cytokinin and auxin. To investigate the ecological relevance of the waterborne bacterial morphogens, seawater samples were collected in the Ria Formosa lagoon (Algarve, Southern Portugal) at 20 sampling sites and tidal pools to assess their morphogenetic effects on the axenic gametes of U. mutabilis. Specifically the survey revealed that sterile-filtered seawater samples can completely recover growth and morphogenesis of U. mutabilis under axenic conditions. Morphogenetic activities of free-living and epiphytic bacteria isolated from the locally very abundant Ulva species (i.e., U. rigida) were screened using a multiwell-based testing system. The most represented genera isolated from U. rigida were Alteromonas, Pseudoalteromonas and Sulfitobacter followed by Psychrobacter and Polaribacter. Several naturally occurring bacterial species could emulate MS2 activity (= induction of cell divisions) regardless of taxonomic affiliation, whereas the MS6 activity (= induction of cell differentiation and cell wall formation) was species-specific and is probably a feature of difficult-to-culture bacteria. Interestingly, isolated bacteroidetes such as Algoriphagus sp. and Polaribacter sp. could individually trigger complete Ulva morphogenesis and thus provide a novel mode of action for bacterial-induced algal development. This study also highlights that the accumulation of algal growth factors in a shallow water body separated from the open ocean by barrier islands might have strong implications to, for example, the wide usage of natural coastal seawater in algal (land based) aquacultures of Ulva.
dc.identifier.doi10.1371/journal.pone.0146307
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/10400.1/9664
dc.language.isoeng
dc.peerreviewedyes
dc.relationTHE EFFECT OF SEAGRASS HABITAT FRAGMENTATION ON SPATIAL STRUCTURE OF METAPOPULATIONS IN SYNGNATHID SPECIES SEAHORSES AND PIPEFISHES
dc.relation.isbasedonWOS:000367815600037
dc.titleMacroalgal morphogenesis induced by waterborne compounds and bacteria in coastal seawater
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleTHE EFFECT OF SEAGRASS HABITAT FRAGMENTATION ON SPATIAL STRUCTURE OF METAPOPULATIONS IN SYNGNATHID SPECIES SEAHORSES AND PIPEFISHES
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/FARH/SFRH%2FBPD%2F64990%2F2009/PT
oaire.citation.endPagee0146307
oaire.citation.issue1
oaire.citation.startPagee0146307
oaire.citation.titlePlos One
oaire.citation.volume11
oaire.fundingStreamFARH
person.familyNameEngelen
person.familyNameda Silva Costa
person.givenNameAschwin
person.givenNameRodrigo
person.identifier115920
person.identifier.ciencia-id911A-9A0C-744D
person.identifier.ciencia-id5917-D500-D251
person.identifier.orcid0000-0002-9579-9606
person.identifier.orcid0000-0002-5932-4101
person.identifier.ridM-3432-2013
person.identifier.ridN-7274-2013
person.identifier.scopus-author-id6701622770
person.identifier.scopus-author-id7203063627
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccess
rcaap.typearticle
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relation.isAuthorOfPublication4495127c-16f2-4231-9fc1-b471c661036b
relation.isAuthorOfPublication.latestForDiscovery33d5a223-f2c9-4c68-984f-9213f15a05b0
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