Headspace-SPME of in vitro shoot-cultures and micropropagated plants of Lavandula viridis

Gonçalves, Sandra; Serra, Hugo; Nogueira, José Manuel F.; Romano, Anabela

http://hdl.handle.net/10400.1/2562

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Authors

Gonçalves, Sandra

Serra, Hugo

Nogueira, José Manuel F.

Romano, Anabela

Abstract(s)

In this work the volatiles emitted from in vitro shoot-cultures and micropropagated plants of Lavandula viridis L’Hér. were characterized and compared with those obtained from the field-grown mother-plant, using headspace solid phase micro-extraction following by capillary gas chromatography coupled to mass spectrometry (HS-SPME-GC/MS). The headspace composition consisted mainly in oxygenated monoterpenes (66.7 - 79.2 %), where the major constituents emitted by the mature field-grown mother-plant, in vitro shoot-cultures and micropropagated plants were 1,8-cineole (74.0, 51.9 and 57.8 %) and camphor (2.9, 15.3 and 8.7 %), respectively. The headspace of in vitro shoot-cultures and micropropagated plants showed greater amount of α-pinene, camphene, β-pinene, β-selinene and selina-3,7(11)-diene, when compared with the field-grown mother-plant.

Keywords

Capillary GC/MS 1,8-cineole Fragrances Lavender Micropropagation Volatiles

URI

http://hdl.handle.net/10400.1/2562

Citation

Gonçalves S., Serra H., Nogueira J. M. F, Almeida R., Custódio L., Romano A. 2008. Headspace-SPME of in vitro shoot-cultures and micropropagated plants of Lavandula viridis, Biologia Plantarum 52: 133-136