Repository logo
 
Publication

Temporal metabolic profiling of the Quercus suber-phytophthora cinnamomi system by middle-infrared spectroscopy

2016-04Journal article Restricted access
Simple item page
dc.contributor.authorHardoim, P.R.
dc.contributor.authorGuerra, Rui Manuel Farinha das Neves
dc.contributor.authorRosa Da Costa, Ana
dc.contributor.authorSerrano, M. S.
dc.contributor.authorSanchez, M. E.
dc.contributor.authorCoelho, A. C.
dc.date.accessioned2017-04-07T15:56:51Z
dc.date.available2017-04-07T15:56:51Z
dc.date.issued2016-04
dc.description.abstractThe oomycete Phytophthora cinnamomi is an aggressive plant pathogen, detrimental to many ecosystems including cork oak (Quercus suber) stands, and can inflict great losses in one of the greatest hotspots' for biodiversity in the world. Here, we applied Fourier transform-infrared (FT-IR) spectroscopy combined with chemometrics to disclose the metabolic patterns of cork oak roots and P.cinnamomi mycelium during the early hours of the interaction. As early as 2h post-inoculation (hpi), cork oak roots showed altered metabolic patterns with significant variations for regions associated with carbohydrate, glycoconjugate and lipid groups when compared to mock-inoculated plants. These variations were further extended at 8hpi. Surprisingly, at 16hpi, the metabolic changes in inoculated and mock-inoculated plants were similar, and at 24hpi, the metabolic patterns of the regions mentioned above were inverted when compared to samples collected at 8hpi. Principal component analysis of the FT-IR spectra confirmed that the metabolic patterns of inoculated cork oak roots could be readily distinguished from those of mock-inoculated plants at 2, 8 and 24hpi, but not at 16hpi. FT-IR spectral analysis from mycelium of P.cinnamomi exposed to cork oak root exudates revealed contrasting variations for regions associated with protein groups at 16 and 24h post-exposure (hpe), whereas carbohydrate and glycoconjugate groups varied mainly at 24hpe. Our results revealed early alterations in the metabolic patterns of the host plant when interacting with the biotrophic pathogen. In addition, the FT-IR technique can be successfully applied to discriminate infected cork oak plants from mock-inoculated plants, although these differences were dynamic with time. To a lesser extent, the metabolic patterns of P.cinnamomi were also altered when exposed to cork oak root exudates.
dc.description.sponsorshipAGL2009-12243-CO2-01
dc.identifier.doi10.1111/efp.12229
dc.identifier.issn1437-4781
dc.identifier.urihttp://hdl.handle.net/10400.1/9545
dc.language.isoeng
dc.peerreviewedyes
dc.relationOMIC METABOLOMICS AND TRANSCRIPTOMICS APPROACHES TO UNRAVEL THE MOLECULAR MECHANISMS OF THE QUERCUS SUBER-PHYTOPHTHORA CINNAMOMI INTERACTION
dc.relationStrategic Project - UI 4023 - 2011-2012
dc.relation.isbasedonWOS:000374015800004
dc.titleTemporal metabolic profiling of the Quercus suber-phytophthora cinnamomi system by middle-infrared spectroscopy
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleOMIC METABOLOMICS AND TRANSCRIPTOMICS APPROACHES TO UNRAVEL THE MOLECULAR MECHANISMS OF THE QUERCUS SUBER-PHYTOPHTHORA CINNAMOMI INTERACTION
oaire.awardTitleStrategic Project - UI 4023 - 2011-2012
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F78931%2F2011/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/PEst-OE%2FQUI%2FUI4023%2F2011/PT
oaire.citation.endPage133
oaire.citation.issue2
oaire.citation.startPage122
oaire.citation.titleForest Pathology
oaire.citation.volume46
oaire.fundingStream6817 - DCRRNI ID
person.familyNameHardoim
person.familyNameGuerra
person.familyNameRosa da Costa
person.givenNamePablo
person.givenNameRui
person.givenNameAna M
person.identifier305029
person.identifier.ciencia-id3D16-5067-D6BB
person.identifier.ciencia-idA418-A85E-5DB1
person.identifier.orcid0000-0002-5079-4959
person.identifier.orcid0000-0002-8642-5792
person.identifier.orcid0000-0003-0225-9537
person.identifier.ridM-7048-2013
person.identifier.ridE-2165-2012
person.identifier.scopus-author-id24173200900
person.identifier.scopus-author-id53986075100
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccess
rcaap.typearticle
relation.isAuthorOfPublication41ce75a1-46ff-4d63-84bc-f4b4700e1b46
relation.isAuthorOfPublicationeff7071e-e676-465b-bae3-983f522acd98
relation.isAuthorOfPublication0ca8c16f-6881-4662-bafc-cc51c5ce8d88
relation.isAuthorOfPublication.latestForDiscovery0ca8c16f-6881-4662-bafc-cc51c5ce8d88
relation.isProjectOfPublication75ae4651-017c-4d61-9653-96afa9184ac4
relation.isProjectOfPublicationb1adbc46-7371-4ed4-a74e-a181b403ca4d
relation.isProjectOfPublication.latestForDiscoveryb1adbc46-7371-4ed4-a74e-a181b403ca4d

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
9545.pdf
Size:
1.22 MB
Format:
Adobe Portable Document Format
Download

Collections

FCT2-Artigos (em revistas ou actas indexadas)