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Interaction of [(VO)-O-IV(acac)(2)] with Human Serum Transferrin and Albumin

2017-08Journal article Open access
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dc.contributor.authorCorreia, Isabel
dc.contributor.authorChorna, Ielyzaveta
dc.contributor.authorCavaco, Isabel
dc.contributor.authorRoy, Somnath
dc.contributor.authorKuznetsov, Maxim L.
dc.contributor.authorRibeiro, Nadia
dc.contributor.authorJustino, Goncalo
dc.contributor.authorMarques, Fernanda
dc.contributor.authorSantos-Silva, Teresa
dc.contributor.authorSantos, Marino F. A.
dc.contributor.authorHugo, M. Santos E.
dc.contributor.authorCapelo, Jose L.
dc.contributor.authorDoutch, James
dc.contributor.authorPessoa, Joao Costa
dc.date.accessioned2019-11-20T15:07:25Z
dc.date.available2019-11-20T15:07:25Z
dc.date.issued2017-08
dc.description.abstractVO(acac)(2)] is a remarkable vanadium compound and has potential as a therapeutic drug. It is important to clarify how it is transported in blood, but the reports addressing its binding to serum proteins have been contradictory. We use several spectroscopic and mass spectrometric techniques (ESI and MALDI-TOF), small-angle X-ray scattering and size exclusion chromatography (SEC) to characterize solutions containing [VO(acac)(2)] and either human serum apotransferrin (apoHTF) or albumin (HSA). DFT and modeling protein calculations are carried out to disclose the type of binding to apoHTF. The measured circular dichroism spectra, SEC and MALDI-TOF data clearly prove that at least two VOacac moieties may bind to apoHTF, most probably forming [(VO)-O-IV(acac)(apoHTF)] complexes with residues of the HTF binding sites. No indication of binding of [VO(acac)(2)] to HSA is obtained. We conclude that (VO)-O-IV-acac species may be transported in blood by transferrin. At very low complex concentrations speciation calculations suggest that [(VO)(apoHTF)] species form.
dc.description.sponsorshipFundacao para a Ciencia e Tecnologia (FCT), Portugal [ RECI/QEQMED/0330/2012, PTDC/QEQ-MED/1902/2014]
dc.description.sponsorshipFCT [IF/00100/2013, IF/00007/2015]
dc.description.sponsorshipPROTEOMASS Scientific Society
dc.description.sponsorshipUCIBIO, Unidade de Ciencias Biomoleculares Aplicadas [UID/Multi/04378/2013]
dc.description.sponsorshipERDF [POCI-01-0145-FEDER-007728, POCI-01-0145-FEDER-007265]
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.doi10.1002/asia.201700469
dc.identifier.issn1861-4728
dc.identifier.issn1861-471X
dc.identifier.urihttp://hdl.handle.net/10400.1/13033
dc.language.isoeng
dc.peerreviewedyes
dc.publisherWiley
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectHeteroleptic oxidovanadium(Iv) compounds
dc.subjectAntidiabetic vanadium complexes
dc.subjectNuclear double-resonance
dc.subjectRay solution scattering
dc.subjectRed-blood-cells
dc.subjectCrystal-structure
dc.subjectOxovanadium(Iv) complexes
dc.subjectN-lobe
dc.subjectTyrosine phosphorylation
dc.subjectCoordination chemistry
dc.titleInteraction of [(VO)-O-IV(acac)(2)] with Human Serum Transferrin and Albumin
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F04349%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FQUI%2F00100%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/RECI%2FQEQ-QIN%2F0189%2F2012/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F77894%2F2011/PT
oaire.citation.endPage2084
oaire.citation.issue16
oaire.citation.startPage2062
oaire.citation.titleChemistry - An Asian Journal
oaire.citation.volume12
oaire.fundingStream5876
oaire.fundingStream5876
oaire.fundingStream3599-PPCDT
oaire.fundingStreamSFRH
person.familyNamePALMA ANTUNES CAVACO
person.givenNameISABEL MARIA
person.identifierH-9087-2012
person.identifier.ciencia-idD515-FB5E-1205
person.identifier.orcid0000-0001-8651-9054
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccess
rcaap.typearticle
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relation.isAuthorOfPublication.latestForDiscoveryb499959b-bea5-4292-bdb2-4311b0aa82de
relation.isProjectOfPublication28685ae5-8021-47a9-948e-d3532f171e8c
relation.isProjectOfPublication9afed05e-58cb-478e-acc7-625439f9c745
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