Repository logo
 
Publication

Membrane and nuclear estrogen receptors in sea bass provide insight to explore genomic and non-genomic estrogenic actions: the mineralized scale example

2017Conference object Open access
Simple item page
dc.contributor.authorPinto, Patricia IS
dc.contributor.authorAndrade, André
dc.contributor.authorFélix, Rute
dc.contributor.authorCardoso, João CR
dc.contributor.authorD Estêvão, M
dc.contributor.authorPower, Deborah
dc.date.accessioned2020-01-30T09:37:41Z
dc.date.available2020-01-30T09:37:41Z
dc.date.issued2017
dc.description.abstractThe numerous estrogen functions across vertebrates have been classically explained by binding to nuclear estrogen receptors (ERs) regulating the transcription of responsive genes. It is now known that estrogenic compounds can also produce rapid non-genomic actions initiated by binding to plasma estrogen membrane receptors, such as the recently identified G protein-coupled estrogen receptor1 (GPER). Sea bass (Dicentrarchus labrax) express three ER subtype genes, one esr1 and two esr2 genes that appear to have been originated from the original esr2 gene in the teleost-specific whole genome duplication. We have recently identified two genes for GPER in the sea bass genome and phylogenetic analyses also suggest they are teleost-specific gene duplicates. Quantitative PCR revealed a wide tissue distribution for the five receptors in both male and female sea bass and expression throughout the reproductive cycle in brain and pituitary, although with subtype-specific and seasonal differences. When analyzing the sea bass scales, mineralized structures previously shown to be estrogen-responsive, a different receptor repertoire and regulation was detected compared to liver, a classical target gene. In juvenile sea bass scales, the main forms expressed were esr2a and gperb, which were also up regulated after injection with the natural estrogen estradiol (E2) and the phytoestrogen genistein (Gen). Both rapid (30 min) and slow (1 day or more) changes in the activities of enzymes related to mineral turnover were detected in fish scales in response to E2, Gen and xenoestrogens and the gene networks activated 1-5 days after injection of E2 and Gen are being characterized by transcriptomics, revealing both common and compound-specific effects at the transcriptional level. Functional characterization of the three sea bass ER subtypes and two GPERs is underway in mammalian cells, to allow to compare their signaling to different estrogenic compounds. These studies will help to understand the normal estrogen regulation of fish scale functions as well as its possible disruption by phytoestrogens and other xenoestrogens and the relative importance of genomic and non-genomic mechanisms of action of the five receptors.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationPinto, P. I. S., Andrade, A., Félix, R. Cardoso, J. C. Estêvão, M. D., Power, D. M. (2017). Membrane and nuclear estrogen receptors in sea bass provide insight to explore genomic and non-genomic estrogenic actions: the mineralized scale example. 18th International Congress of Comparative Endocrinology, Lake Louise, Canada, 4-9/6/2017.pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.1/13441
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationE2SCALE: Scaling-down estrogenic responses in fish
dc.relationCentre of Marine Sciences
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.titleMembrane and nuclear estrogen receptors in sea bass provide insight to explore genomic and non-genomic estrogenic actions: the mineralized scale examplept_PT
dc.typeconference object
dspace.entity.typePublication
oaire.awardTitleE2SCALE: Scaling-down estrogenic responses in fish
oaire.awardTitleCentre of Marine Sciences
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FAAG-GLO%2F4003%2F2012/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FMulti%2F04326%2F2013/PT
oaire.citation.conferencePlaceLake Louisept_PT
oaire.citation.title18th International Congress of Comparative Endocrinologypt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStream6817 - DCRRNI ID
person.familyNamePinto
person.familyNameAlçada Baptista Rebelo de Andrade
person.familyNameFélix
person.familyNameCardoso
person.familyNameEstêvão
person.familyNamePower
person.givenNamePatricia IS
person.givenNameAndré
person.givenNameRute
person.givenNameJoão
person.givenNameMaria Dulce da Mota Antunes de Oliveira
person.givenNameDeborah Mary
person.identifier643457
person.identifier14332
person.identifierI-4695-2014
person.identifier.ciencia-idE51D-5CCB-B1C6
person.identifier.ciencia-idF513-56ED-7088
person.identifier.ciencia-idFB1A-1325-8E25
person.identifier.ciencia-id8B16-F203-2AFC
person.identifier.ciencia-id881B-53E8-0DB1
person.identifier.ciencia-id891A-8A44-3CAE
person.identifier.orcid0000-0001-7854-3898
person.identifier.orcid0000-0002-5900-8782
person.identifier.orcid0000-0002-4144-3452
person.identifier.orcid0000-0001-7890-0170
person.identifier.orcid0000-0002-7151-8363
person.identifier.orcid0000-0003-1366-0246
person.identifier.ridM-3817-2013
person.identifier.ridM-6164-2013
person.identifier.ridM-4151-2013
person.identifier.scopus-author-id10240774300
person.identifier.scopus-author-id56006773000
person.identifier.scopus-author-id7201822956
person.identifier.scopus-author-id8381590400
person.identifier.scopus-author-id7101806760
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
relation.isAuthorOfPublication64bc526e-5281-44e5-91eb-5743436109f9
relation.isAuthorOfPublication44cc56db-1879-412b-86eb-8ce5465a427a
relation.isAuthorOfPublication9b5e820d-e41f-4c28-8832-823658d29ae9
relation.isAuthorOfPublication1b670c84-15e3-4776-8871-50f9eb0eed0d
relation.isAuthorOfPublication67887883-2695-48cc-8221-aa46780ac143
relation.isAuthorOfPublicationc68f5ffb-63f6-4c70-8957-29e464fb59c0
relation.isAuthorOfPublication.latestForDiscoveryc68f5ffb-63f6-4c70-8957-29e464fb59c0
relation.isProjectOfPublication66289c1f-16cd-4d25-b30f-1eb63d5ecd80
relation.isProjectOfPublication42f9a7c8-8d5c-41d5-b490-9bb0334ea269
relation.isProjectOfPublication.latestForDiscovery66289c1f-16cd-4d25-b30f-1eb63d5ecd80

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
PPinto poster ICCE_2017_FINAL.pdf
Size:
4.92 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
3.46 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

ESS4-Vários
CCM4-Vários