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Rewired glycosylation activity promotes scarless regeneration and functional recovery in spiny mice after complete spinal cord transection

2022Journal article Restricted
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dc.contributor.authorNogueira-Rodrigues, Joana
dc.contributor.authorLeite, Sérgio C.
dc.contributor.authorPinto-Costa, Rita
dc.contributor.authorSousa, Sara C.
dc.contributor.authorLuz, Liliana L.
dc.contributor.authorSintra, Maria A.
dc.contributor.authorOliveira, Raquel
dc.contributor.authorMonteiro, Ana C.
dc.contributor.authorPinheiro, Gonçalo
dc.contributor.authorVitorino, Marta
dc.contributor.authorSilva, Joana A.
dc.contributor.authorS, Simão
dc.contributor.authorVitor Fernandes, Dr
dc.contributor.authorProvazník, Jan
dc.contributor.authorBenes, Vladimir
dc.contributor.authorCruz, Célia D.
dc.contributor.authorSafronov, Boris V.
dc.contributor.authorMagalhães, Ana
dc.contributor.authorReis, Celso A.
dc.contributor.authorVieira, Jorge
dc.contributor.authorVieira, Cristina P.
dc.contributor.authorTiscórnia, Gustavo
dc.contributor.authorAraujo, Ines
dc.contributor.authorSousa, Mónica M.
dc.date.accessioned2023-03-09T17:29:33Z
dc.date.available2023-03-09T17:29:33Z
dc.date.issued2022
dc.description.abstractRegeneration of adult mammalian central nervous system (CNS) axons is abortive, resulting in inability to recover function after CNS lesion, including spinal cord injury (SCI). Here, we show that the spiny mouse (Acomys) is an exception to other mammals, being capable of spontaneous and fast restoration of function after severe SCI, re-establishing hind limb coordination. Remarkably, Acomys assembles a scarless pro-regenerative tissue at the injury site, providing a unique structural continuity of the initial spinal cord geometry. The Acomys SCI site shows robust axon regeneration of multiple tracts, synapse formation, and electrophysiological signal propagation. Transcriptomic analysis of the spinal cord following transcriptome reconstruction revealed that Acomys rewires glycosylation biosynthetic pathways, culminating in a specific pro-regenerative proteoglycan signature at SCI site. Our work uncovers that a glycosylation switch is critical for axon regeneration after SCI and identifies beta 3gnt7, a crucial enzyme of keratan sulfate biosynthesis, as an enhancer of axon growth.pt_PT
dc.description.sponsorshipSanta Casa da Misericordia de Lisboa MC-39-2019, Wings for Life WFL-PT-21/20, Portuguese Foundation for Science and Technology UID/BIM/04773/2013/CBMRpt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.devcel.2021.12.008pt_PT
dc.identifier.eissn1878-1551
dc.identifier.urihttp://hdl.handle.net/10400.1/19217
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherCell Presspt_PT
dc.relationCentre for Biomedical Research
dc.relationRegeneration in the African Spiny Mouse
dc.relationIdentifying molecules and mechanisms enhancing axon growth and antagonizing axon growth inhibition: a combined approach
dc.relationSTRETCH: Dissecting the molecular mechanisms underlying tension-driven axon growth
dc.subjectPlasticitypt_PT
dc.subjectMousept_PT
dc.titleRewired glycosylation activity promotes scarless regeneration and functional recovery in spiny mice after complete spinal cord transectionpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleCentre for Biomedical Research
oaire.awardTitleRegeneration in the African Spiny Mouse
oaire.awardTitleIdentifying molecules and mechanisms enhancing axon growth and antagonizing axon growth inhibition: a combined approach
oaire.awardTitleSTRETCH: Dissecting the molecular mechanisms underlying tension-driven axon growth
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FBIM%2F04773%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/9471 - RIDTI/PTDC%2FMED-ONC%2F28489%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FBIA-ANM%2F0697%2F2014/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F131565%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F136760%2F2018/PT
oaire.citation.endPage450.e7pt_PT
oaire.citation.issue4pt_PT
oaire.citation.startPage440pt_PT
oaire.citation.titleDevelopmental Cellpt_PT
oaire.citation.volume57pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream9471 - RIDTI
oaire.fundingStream3599-PPCDT
person.familyNameGoulart da Silva Pinheiro
person.familyNameVitorino
person.familyNameSimao
person.familyNameE. Fernandes
person.familyNamePombinho de Araújo
person.givenNameGonçalo
person.givenNameMarta
person.givenNameSonia
person.givenNameVitor
person.givenNameInês Maria
person.identifierF-4703-2012
person.identifier.ciencia-idFC1A-C7E5-EE1A
person.identifier.ciencia-id4615-FF5B-E951
person.identifier.ciencia-id3811-AC60-F24D
person.identifier.ciencia-idD11F-D4CA-2947
person.identifier.orcid0000-0001-8563-8975
person.identifier.orcid0000-0002-0245-0633
person.identifier.orcid0000-0001-5179-2122
person.identifier.orcid0000-0002-2438-0111
person.identifier.ridU-9947-2018
person.identifier.scopus-author-id24067982400
person.identifier.scopus-author-id36155199000
person.identifier.scopus-author-id56271084100
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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