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Genotyping and susceptibility assessment of bacteria of the ENTEROBACTERIALES isolated from patients suffering from fecal incontinence
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Abstract(s)
The human intestinal microbiota is composed of large number of microorganisms that reside in our intestine. This community is mainly constituted by a great diversity of bacteria, fungi and bacteriophages. Amidst them, a large proportion of bacteria that live in the gastrointestinal tract are bacteria that belong to the order of Enterobacteriales. When the community of the members of the order Enterobacteriales unbalanced, they can lead to intestinal dysbiosis that can impact the health status of the host. The alteration in the bacterial community of human intestine can be associate to many factors, for instance unproper use of antibiotics, which change the intestinal bacteriome equilibrium. The results can include inflamed intestine, faecal incontinence (FI). The goal of the current study was to isolate and identify members of the order Enterobacteriales followed by their genotyping BOX-PCR and determine their antibiotic susceptibility. For this purpose, 25 faecal samples of women over 60 years that suffered from faecal incontinence that attended in hospitals in the Algarve region. From these 25 samples analyzed, 72 isolates were obtained, which identification was performed using the Remel RapID ONE System. Escherichia Coli, Shigella sp., Klebsiella pneumoniae, and E. coli 0157:H7 were the more prevalent among the samples. Regarding the BOX-PCR profile the bacterial isolates showed a large diversity. Moreover, 80 to 94,7% of similarity was observed between 27 clusters, unveiling high degree of enterobacterial diversity among the isolate. It is important to highlight that only 3 faecal samples showed to carry specific strains. The antibiotic susceptibility was evaluated by using Kirby-Bauer disk diffusion method. The resistance profiles were observed for amoxicillin (AMC), with 72, 20% among the 72 isolates. the Multidrug Resistance profile (MDR) was more frequent for penicillins than other antibiotic class with 79% of MDR. The multidrug resistance observed among these samples will challenge the treatment of the patients. The BOX-PCR technique showed to good distinction between the bacterial genus and species, unfortunately it was no able to discriminate variants in the same species. Thus, more effective genomic studies are require.
The human intestinal microbiota is composed of large number of microorganisms that reside in our intestine. This community is mainly constituted by a great diversity of bacteria, fungi and bacteriophages. Amidst them, a large proportion of bacteria that live in the gastrointestinal tract are bacteria that belong to the order of Enterobacteriales. When the community of the members of the order Enterobacteriales unbalanced, they can lead to intestinal dysbiosis that can impact the health status of the host. The alteration in the bacterial community of human intestine can be associate to many factors, for instance unproper use of antibiotics, which change the intestinal bacteriome equilibrium. The results can include inflamed intestine, faecal incontinence (FI). The goal of the current study was to isolate and identify members of the order Enterobacteriales followed by their genotyping BOX-PCR and determine their antibiotic susceptibility. For this purpose, 25 faecal samples of women over 60 years that suffered from faecal incontinence that attended in hospitals in the Algarve region. From these 25 samples analyzed, 72 isolates were obtained, which identification was performed using the Remel RapID ONE System. Escherichia Coli, Shigella sp., Klebsiella pneumoniae, and E. coli 0157:H7 were the more prevalent among the samples. Regarding the BOX-PCR profile the bacterial isolates showed a large diversity. Moreover, 80 to 94,7% of similarity was observed between 27 clusters, unveiling high degree of enterobacterial diversity among the isolate. It is important to highlight that only 3 faecal samples showed to carry specific strains. The antibiotic susceptibility was evaluated by using Kirby-Bauer disk diffusion method. The resistance profiles were observed for amoxicillin (AMC), with 72, 20% among the 72 isolates. the Multidrug Resistance profile (MDR) was more frequent for penicillins than other antibiotic class with 79% of MDR. The multidrug resistance observed among these samples will challenge the treatment of the patients. The BOX-PCR technique showed to good distinction between the bacterial genus and species, unfortunately it was no able to discriminate variants in the same species. Thus, more effective genomic studies are required.
The human intestinal microbiota is composed of large number of microorganisms that reside in our intestine. This community is mainly constituted by a great diversity of bacteria, fungi and bacteriophages. Amidst them, a large proportion of bacteria that live in the gastrointestinal tract are bacteria that belong to the order of Enterobacteriales. When the community of the members of the order Enterobacteriales unbalanced, they can lead to intestinal dysbiosis that can impact the health status of the host. The alteration in the bacterial community of human intestine can be associate to many factors, for instance unproper use of antibiotics, which change the intestinal bacteriome equilibrium. The results can include inflamed intestine, faecal incontinence (FI). The goal of the current study was to isolate and identify members of the order Enterobacteriales followed by their genotyping BOX-PCR and determine their antibiotic susceptibility. For this purpose, 25 faecal samples of women over 60 years that suffered from faecal incontinence that attended in hospitals in the Algarve region. From these 25 samples analyzed, 72 isolates were obtained, which identification was performed using the Remel RapID ONE System. Escherichia Coli, Shigella sp., Klebsiella pneumoniae, and E. coli 0157:H7 were the more prevalent among the samples. Regarding the BOX-PCR profile the bacterial isolates showed a large diversity. Moreover, 80 to 94,7% of similarity was observed between 27 clusters, unveiling high degree of enterobacterial diversity among the isolate. It is important to highlight that only 3 faecal samples showed to carry specific strains. The antibiotic susceptibility was evaluated by using Kirby-Bauer disk diffusion method. The resistance profiles were observed for amoxicillin (AMC), with 72, 20% among the 72 isolates. the Multidrug Resistance profile (MDR) was more frequent for penicillins than other antibiotic class with 79% of MDR. The multidrug resistance observed among these samples will challenge the treatment of the patients. The BOX-PCR technique showed to good distinction between the bacterial genus and species, unfortunately it was no able to discriminate variants in the same species. Thus, more effective genomic studies are required.
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Keywords
Faecal incontinence intestinal microbiota Enterobacterales antibiotic resistance