Rapid clonal multiplication of Lavandula viridis L'Her through in vitro axillary shoot proliferation

Dias, M. C.; Almeida, R.; Romano, Anabela

http://hdl.handle.net/10400.1/6416

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Authors

Dias, M. C.

Almeida, R.

Romano, Anabela

Abstract(s)

In vitro clonal propagation of native Mediterranean Lavandula viridis was obtained from a mature field-grown plant. Single node explants were successfully established on Murashige and Skoog medium supplemented with 0.44 muM of 6-benzyladenine. The highest multiplication rate (11.69 shoots/node) was obtained with 0.67 muM 6-benzyladenine in Murashige and Skoog medium with macronutrients at half-strength. Shoots were easily rooted on Gresshoff and Doy medium. Increasing sucrose concentration from 58.4 to 87.6 mM resulted in a significant increase in rooting frequency. Eighty per cent of plantlets were successfully acclimatised to ex vitro conditions, exhibiting a normal development.