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Olfactory sensitivity to changes in environmental [Ca2+] in the marine teleost Sparus aurata

2000-12-15Journal article Open access
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dc.contributor.authorHubbard, Peter
dc.contributor.authorBarata, E. N.
dc.contributor.authorCanario, Adelino V. M.
dc.date.accessioned2014-05-09T17:41:01Z
dc.date.available2014-05-09T17:41:01Z
dc.date.issued2000-12-15
dc.date.updated2014-05-07T11:34:00Z
dc.description.abstractEstuarine and/or migratory teleosts may experience large and rapid changes in external [Ca2+]. Previous studies have largely centred on the physiological mechanisms that maintain a constant plasma [Ca2+] in the face of such external fluctuations, but little work has been directed to examining how these changes may originally be detected. We present evidence that the olfactory system of the gilthead seabream (Sparus aurata) is highly sensitive to reductions in environmental [Ca2+] and suggest a possible mechanism by which this may be mediated. Multi-unit extracellular recordings were made from the olfactory nerve of Sparus aurata while the [Ca2+] of artificial sea water flowing over the olfactory epithelium was varied from 10 to 0mmoll-1. Reductions in [Ca2+] caused a large, nonaccommodating increase in the firing rate of the olfactory nerve (apparent IC50=1.67±0.26mmoll-1, apparent Hill coefficient=-1.22±0.14; means ± S.E.M., N=6). This response was not due to the concomitant reduction in osmolality and was specific for Ca2+. During continuous exposure of the olfactory epithelium to Ca2+-free sea water, the apparent IC50 and Hill coefficient in response to increases in [Ca2+] were 0.48±0.14mmoll-1 and -0.76±0.16 (means ± S.E.M., N=6), respectively, suggesting an adaptation of the Ca2+- sensing system to low-[Ca2+] environments. Ca2+ is intimately involved in signal transduction in the olfactory receptor neurones, but our data support a true olfactory response, rather than a non-specific effect to lowering of external [Ca2+]. The absence of Ca2+ from sea water only partially and temporarily blunted the olfactory response to the odorant L-serine; the response amplitude recovered to control levels within 20 min. This suggests that the olfactory system in general is able to adapt to low-[Ca2+] environments. We suggest that the Ca2+ sensitivity is mediated by an extracellular Ca2+-sensing receptor similar to the recently characterized mammalian Ca2+-sensing receptor.por
dc.identifier.citationHubbard, P. C.; Barata, E. N.; Canario, A. V. M.Olfactory sensitivity to changes in environmental [Ca2+] in the marine teleost Sparus aurata, The Journal of Experimental Biology, 203, 24, 3821-3829, 2000.por
dc.identifier.issn0022-0949
dc.identifier.otherAUT: ACA00258
dc.identifier.urihttp://hdl.handle.net/10400.1/3846
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherThe Company of Biologistspor
dc.relation.publisherversionhttp://jeb.biologists.org/content/203/24/3821.full.pdf+htmlpor
dc.subjectCa2+-sensing receptorpor
dc.subjectTeleostpor
dc.subjectMarinepor
dc.subjectOlfactionpor
dc.titleOlfactory sensitivity to changes in environmental [Ca2+] in the marine teleost Sparus auratapor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage3829por
oaire.citation.issue24por
oaire.citation.startPage3821por
oaire.citation.titleThe Journal of Experimental Biologypor
oaire.citation.volume203por
person.familyNameHubbard
person.familyNameBarata
person.familyNameCanario
person.givenNamePeter
person.givenNameEduardo Nuno
person.givenNameAdelino
person.identifier143624
person.identifier.ciencia-id951F-BA4B-A21F
person.identifier.ciencia-id1F1E-D3B3-F804
person.identifier.orcid0000-0002-3007-4647
person.identifier.orcid0000-0001-9714-5967
person.identifier.orcid0000-0002-6244-6468
person.identifier.ridB-2823-2008
person.identifier.ridB-2902-2010
person.identifier.ridC-7942-2009
person.identifier.scopus-author-id7005928467
person.identifier.scopus-author-id6602073256
person.identifier.scopus-author-id56568523700
rcaap.rightsopenAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublicationf599b3ac-806b-4f65-9eb6-f70e68bcb142
relation.isAuthorOfPublicationa2bb6982-e4c8-4ea3-b160-bc3464cd960f
relation.isAuthorOfPublication5f6e51ee-9113-469e-8b9e-f30f2d452521
relation.isAuthorOfPublication.latestForDiscovery5f6e51ee-9113-469e-8b9e-f30f2d452521

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