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Extrusion of benzoic acid in Saccharomyces cerevisiae by an energy-dependent mechanism

dc.contributor.authorHenriques, M.
dc.contributor.authorQuintas, Célia
dc.contributor.authorLoureiro-Dias, M. C.
dc.date.accessioned2015-12-03T14:46:01Z
dc.date.available2015-12-03T14:46:01Z
dc.date.issued1997
dc.description.abstractWhen grown in the presence of benzoic acid, Saccharomyces cerevisiae was able to extrude [C-14]benzoic acid when a pulse of glucose was given to preloaded cells. While octanoic, sorbic, hexanoic, salicylic, butyric and propionic acids were also inducers, ethanol and acetic acid were not. The mechanism of extrusion required energy and prior growth in the presence of the inducers. Diethylstilbestrol, an inhibitor of ATPases, prevented benzoic acid extrusion. Propionic acid was not actively extruded in cells adapted to either benzoic or propionic acid, behaving as an appropriate probe to measure intracellular ph. Even though the extrusion mechanism was active, benzoic acid entered the cells by a simple diffusion mechanism.
dc.identifier.issn1350-0872
dc.identifier.otherAUT: CQU00490;
dc.identifier.urihttp://hdl.handle.net/10400.1/7301
dc.language.isoeng
dc.peerreviewedyes
dc.publisherMicrobiology Society
dc.relation.isbasedonP-001-BBZ
dc.titleExtrusion of benzoic acid in Saccharomyces cerevisiae by an energy-dependent mechanism
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage1883
oaire.citation.startPage1877
oaire.citation.titleMicrobiology
oaire.citation.volume143
person.familyNameQuintas
person.givenNameCélia
person.identifier.ciencia-idEA14-D76B-FF6E
person.identifier.orcid0000-0001-9680-5089
rcaap.rightsrestrictedAccess
rcaap.typearticle
relation.isAuthorOfPublication7c06dbb4-eb11-4f22-b0f5-3cf276d919f4
relation.isAuthorOfPublication.latestForDiscovery7c06dbb4-eb11-4f22-b0f5-3cf276d919f4

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