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Sturgeon osteocalcin shares structural features with matrix gla protein evolutionary relationship and functional implications

2013-09Journal article Open access
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dc.contributor.authorS B Viegas, Carla
dc.contributor.authorSimes, Dina
dc.contributor.authorWilliamson, Matthew K.
dc.contributor.authorCavaco, Sofia
dc.contributor.authorLaizé, Vincent
dc.contributor.authorPrice, Paul A.
dc.contributor.authorLeonor Cancela, M.
dc.date.accessioned2018-12-07T14:58:18Z
dc.date.available2018-12-07T14:58:18Z
dc.date.issued2013-09
dc.description.abstractOsteocalcin (OC) and matrix Gla protein (MGP) are considered evolutionarily related because they share key structural features, although they have been described to exert different functions. In this work, we report the identification and characterization of both OC and MGP from the Adriatic sturgeon, a ray-finned fish characterized by a slow evolution and the retention of many ancestral features. Sturgeon MGP shows a primary structure, post-translation modifications, and patterns of mRNA/protein distribution and accumulation typical of known MGPs, and it contains seven possible Gla residues that would make the sturgeon protein the most gamma-carboxylated among known MGPs. In contrast, sturgeon OC was found to present a hybrid structure. Indeed, although exhibiting protein domains typical of known OCs, it also contains structural features usually found in MGPs (e. g. a putative phosphorylated propeptide). Moreover, patterns of OC gene expression and protein accumulation overlap with those reported for MGP; OC was detected in bone cells and mineralized structures but also in soft and cartilaginous tissues. We propose that, in a context of a reduced rate of evolution, sturgeon OC has retained structural features of the ancestral protein that emerged millions of years ago from the duplication of an ancient MGP gene and may exhibit intermediate functional features.
dc.description.sponsorshipPortuguese Science and Technology Foundation [POCTI/MAR/57921/2004, SFRH/BD/9077/2002]; Fundo Europeu De Desenvolvimento Regional (FEDER) (Portugal); National Funding; Center of Marine Sciences (CCMAR)
dc.identifier.doi10.1074/jbc.M113.450213
dc.identifier.issn0021-9258
dc.identifier.urihttp://hdl.handle.net/10400.1/11950
dc.language.isoeng
dc.peerreviewedyes
dc.publisherAmerican Society for Biochemistry and Molecular Biology
dc.subjectGamma-carboxyglutamic acid
dc.subjectFish Sparus-Aurata
dc.subjectVitamin-K
dc.subjectXenopus-Laevis
dc.subjectTeleost fish
dc.subjectCalcified cartilage
dc.subjectTissue distribution
dc.subjectOrganic matrix
dc.subjectMessenger-Rna
dc.subjectBone
dc.titleSturgeon osteocalcin shares structural features with matrix gla protein evolutionary relationship and functional implications
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/POCI/POCI%2FMAR%2F57921%2F2004/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F9077%2F2002/PT
oaire.citation.endPage27811
oaire.citation.issue39
oaire.citation.startPage27801
oaire.citation.titleJournal of Biological Chemistry
oaire.citation.volume288
oaire.fundingStreamPOCI
oaire.fundingStreamSFRH
person.familyNameViegas
person.familyNameSimes
person.familyNameCavaco
person.familyNameLaizé
person.familyNameCancela
person.givenNameCarla
person.givenNameDina
person.givenNameSofia
person.givenNameVincent
person.givenNameM. Leonor
person.identifier.ciencia-idC414-F596-EEC6
person.identifier.ciencia-id9317-E1FC-06F0
person.identifier.orcid0000-0002-5765-3665
person.identifier.orcid0000-0002-5145-4753
person.identifier.orcid0000-0001-7315-4543
person.identifier.orcid0000-0001-9565-9198
person.identifier.orcid0000-0003-3114-6662
person.identifier.ridN-6695-2014
person.identifier.ridN-2789-2014
person.identifier.ridB-4463-2008
person.identifier.scopus-author-id8656310300
person.identifier.scopus-author-id7201884723
person.identifier.scopus-author-id6506957933
person.identifier.scopus-author-id6602982778
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccess
rcaap.typearticle
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