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First study in cryopreserved Crassostrea angulata sperm

2017-05Journal article Restricted access
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dc.contributor.authorF RIESCO, MARTA
dc.contributor.authorFélix, Francisca
dc.contributor.authorMatias, Domitilia
dc.contributor.authorJoaquim, Sandra
dc.contributor.authorSuquet, Marc
dc.contributor.authorCabrita, Elsa
dc.date.accessioned2019-11-20T15:07:40Z
dc.date.available2019-11-20T15:07:40Z
dc.date.issued2017-05
dc.description.abstractSperm cryopreservation is a widely employed technique that promotes alternative techniques to contribute to broodstock management or restoration programs for species of commercial interest, endangered species or species with an interesting genotype. The preservation of genetic material from improved stocks or from the original population is extremely important for the oyster aquaculture industry to prevent the potential impacts of epidemic diseases and natural disasters. The Portuguese oyster, Crassostrea angulata, was the most important species commercialized by the shellfish industry. However, inadequate management of this industry and pathology occurrences resulted in a significant decrease in natural populations. For this reason, in this work a successful sperm cryopreservation protocol for this important species has been developed for the first time. Different internal cryoprotectants (DMSO, ethylene glycol, polyethylene glycol and methanol) at several concentrations (5, 10, 20%), containers (straws vs cryovials) and freezing rates (slow and fast rates) were tested. Cryoprotectant toxicity tests corroborated that this assay did not take into account the following steps of cryopreservation protocol as sperm agglutination. A fast freezing rate of cells diluted in 10% DMSO and the use of straws as containers were the best cryopreservation conditions for Portuguese oyster sperm. Finally, fertilization assays confirmed the efficiency of the cryopreservation protocol in oyster sperm. These results demonstrated that different susceptibilities have been detected concerning sperm cryopreservation depending on oyster species or genetic material composition. (C) 2016 Elsevier Inc. All rights reserved.
dc.description.sponsorshipPromar Program [CRIOBIV-31-03-05-FEP-59]
dc.description.sponsorshipCRIOBIV project
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.doi10.1016/j.ygcen.2016.05.003
dc.identifier.issn0016-6480
dc.identifier.issn1095-6840
dc.identifier.urihttp://hdl.handle.net/10400.1/13153
dc.language.isoeng
dc.peerreviewedyes
dc.publisherAcademic Press Inc Elsevier Science
dc.subjectPacific oyster
dc.subjectPortuguese oyster
dc.subjectGigas
dc.subjectVirginica
dc.subjectFertilization
dc.subjectSpermatozoa
dc.subjectViability
dc.subjectQuality
dc.subjectOocytes
dc.subjectSemen
dc.titleFirst study in cryopreserved Crassostrea angulata sperm
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage115
oaire.citation.startPage108
oaire.citation.titleGeneral and Comparative Endocrinology
oaire.citation.volume245
person.familyNameF RIESCO
person.familyNameFélix de Azeredo Pinto e Melo
person.familyNameCabrita
person.givenNameMARTA
person.givenNameFrancisca
person.givenNameElsa
person.identifier.ciencia-idAC1C-FB89-E69C
person.identifier.orcid0000-0002-3655-6401
person.identifier.orcid0000-0003-3864-8841
person.identifier.ridO-9766-2014
person.identifier.ridE-8523-2012
person.identifier.scopus-author-id54796976600
person.identifier.scopus-author-id26645697000
rcaap.rightsrestrictedAccess
rcaap.typearticle
relation.isAuthorOfPublication9e0d4fdf-78d3-4eda-85a2-ff13b385449b
relation.isAuthorOfPublication8a5633cd-dc8c-47ef-9d48-838e184d8225
relation.isAuthorOfPublication332fa9b9-09b5-47fe-a1b3-afe088b4150a
relation.isAuthorOfPublication.latestForDiscovery9e0d4fdf-78d3-4eda-85a2-ff13b385449b

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