Repository logo
 
Publication

Calcitonin receptor family evolution and fishing for function using in silico promoter analysis

2014Journal article Restricted access
Simple item page
dc.contributor.authorMartins, R.
dc.contributor.authorVieira, Florbela A.
dc.contributor.authorPower, Deborah
dc.date.accessioned2014-10-15T13:41:28Z
dc.date.available2014-10-15T13:41:28Z
dc.date.issued2014
dc.description.abstractIn the present study the calcitonin receptor (CTR) sub-family of family B G-protein coupled receptors (GPCRs) in teleosts is evaluated and put in the context of the families overall evolution from echinodermates to vertebrates. Echinodermates, hemichordates, cephalochordates and tunicates have a single gene that encodes a receptor that bears similarity to the vertebrate calcitonin receptor (CTR) and calcitoninlike receptor (CTR/CLR). In tetrapods one gene encodes the calcitonin receptor (CALCR) and another gene the calcitonin receptor-like receptor (CALCRL). The evolution of CALCR has been under strong conservative pressure and a single copy is also found in fishes and high conservation of gene organisation and synteny exits from teleosts to human. A teleost specific CTR innovation that occurred after their divergence from holostei is the presence of several HBDs in the N-terminus. CALCRL had a different evolutionary trajectory from CALCR and although a single gene copy is present in tetrapods the sarcopterygii fish, the coelacanth, has 1 copy of CALCRL but also a fish specific form CALCRL3. The ray-finned fish, the spotted gar, has 1 copy of CALCRL and 1 of CALCRL3 but the teleost specific whole genome duplication has resulted in a CALCRL1 and CALCRL2 in addition to the fish specific CALCRL3. Strong conservation of CALCRL gene structure exists from human to fish. Promoter analysis in silico reveals that the duplicated CALCRL genes in the teleosts, zebrafish, takifugu, tetraodon and medaka, have divergent promoters and different putative co-regulated gene partners suggesting their function is different.por
dc.description.sponsorshipWe are grateful to Professor Adelino Canário for providing the sequence of sea bass EST clones. This study was supported by the European Regional Development Fund (ERDF) COMPETE – Operational Competitiveness Programme and Portuguese funds through FCT – Foundation for Science and Technology, under the project ‘‘PEst-C/MAR/LA0015/2013’’ and by FCT PTDC/BIA-BCM//73597/ 2010. RM (SFRH/BPD/66742/2009) and FV (SFRH/BPD/73597/ 2010) were in receipt of a post-doctoral grant from FCT, Portugal.por
dc.identifier.citationR. Martins, F.A. Vieira, D.M. Power, "Calcitonin receptor family evolution and fishing for function using in silico promoter analysis" in General and Comparative Endocrinology, 2014 (in press).por
dc.identifier.doihttp://dx.doi.org/10.1016/j.ygcen.2014.04.025
dc.identifier.issn0016-6480
dc.identifier.otherAUT: DPO00386;
dc.identifier.urihttp://hdl.handle.net/10400.1/5330
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.subjectCalcitoninpor
dc.subjectEvolutionpor
dc.subjectG protein coupled receptorspor
dc.subjectPromoter analysispor
dc.subjectSyntenypor
dc.titleCalcitonin receptor family evolution and fishing for function using in silico promoter analysispor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.titleGeneral and Comparative Endocrinologypor
person.familyNamePower
person.givenNameDeborah Mary
person.identifier.ciencia-id891A-8A44-3CAE
person.identifier.orcid0000-0003-1366-0246
person.identifier.scopus-author-id7101806760
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublicationc68f5ffb-63f6-4c70-8957-29e464fb59c0
relation.isAuthorOfPublication.latestForDiscoveryc68f5ffb-63f6-4c70-8957-29e464fb59c0

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Calcitonin receptor family evolution and fishing for function using in silico promoter analysis.pdf
Size:
3.16 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.61 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

CCM2-Artigos (em revistas ou actas indexadas)