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A blood-free diet to rear anopheline mosquitoes

dc.contributor.authorMarques, Joana
dc.contributor.authorCardoso, João CR
dc.contributor.authorFélix, Rute C.
dc.contributor.authorPower, Deborah
dc.contributor.authorSilveira, Henrique
dc.date.accessioned2020-06-05T11:29:41Z
dc.date.available2020-12-01T01:30:13Z
dc.date.issued2020
dc.description.abstractMalaria research requires large-scale breeding and production conditions for mosquitoes (Anopheles spp.) in captivity. The sustainable and reliable production of mosquitoes is currently inhibited by the supply of fresh vertebrate blood. Alternatives to blood are required to promote efficient control strategies for malaria and other vector borne diseases that are transmitted by blood feeding insects. With this in mind, artificial liquid diets were formulated as substitutes for fresh vertebrate blood. Herein we report a blood-free artificial liquid diet that delivers feeding rates similar to blood and mimics the physiological effects of a fresh vertebrate blood meal. The diet induces ovarian and egg maturation of Anopheles mosquitoes and also produces good larval survival and development of functional adults. The formulated blood-free liquid diet is an important advance towards sustainable mosquito breeding in captivity and will reduce the maintenance costs of mosquito colonies and eliminate the need for fresh vertebrate blood.pt_PT
dc.description.sponsorshipFundaçãopara a Ciência e Tecnologia (CEECIND/00450/2017).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.3791/60144pt_PT
dc.identifier.issn1940-087X
dc.identifier.urihttp://hdl.handle.net/10400.1/13989
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherJournal of Visualized Experimentspt_PT
dc.subjectBiologypt_PT
dc.subjectOogenesispt_PT
dc.subjectIssue 155pt_PT
dc.subjectFresh blood substitutespt_PT
dc.subjectArtificial mealpt_PT
dc.subjectAnopheles dietpt_PT
dc.subjectMosquitopt_PT
dc.subjectRearingpt_PT
dc.titleA blood-free diet to rear anopheline mosquitoespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F04413%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F04326%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F89811%2F2012/PT
oaire.citation.endPage11pt_PT
oaire.citation.issue155pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titleJournal of Visualized Experimentspt_PT
oaire.fundingStream5876
oaire.fundingStream5876
oaire.fundingStreamSFRH
person.familyNameCardoso
person.familyNameFélix
person.familyNamePower
person.givenNameJoão
person.givenNameRute
person.givenNameDeborah Mary
person.identifier14332
person.identifier.ciencia-id8B16-F203-2AFC
person.identifier.ciencia-idFB1A-1325-8E25
person.identifier.ciencia-id891A-8A44-3CAE
person.identifier.orcid0000-0001-7890-0170
person.identifier.orcid0000-0002-4144-3452
person.identifier.orcid0000-0003-1366-0246
person.identifier.ridM-4151-2013
person.identifier.ridM-6164-2013
person.identifier.scopus-author-id7201822956
person.identifier.scopus-author-id56006773000
person.identifier.scopus-author-id7101806760
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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