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Kinetic and energetic parameters of carob wastes fermentation by Saccharomyces cerevisiae: crabtree effect, ethanol toxicity, and invertase repression

2015-06Journal article Open access
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dc.contributor.authorRodrigues, Brígida
dc.contributor.authorPeinado, J. M.
dc.contributor.authorRaposo, Sara
dc.contributor.authorConstantino, Ana
dc.contributor.authorQuintas, Célia
dc.contributor.authorEmília Lima-Costa, Maria
dc.date.accessioned2020-05-22T09:09:41Z
dc.date.available2020-05-22T09:09:41Z
dc.date.issued2015-06
dc.description.abstractCarob waste is a useful raw material for the second-generation ethanol because 50% of its dry weight is sucrose, glucose, and fructose. To optimize the process, we have studied the influence of the initial concentration of sugars on the fermentation performance of Saccharomyces cerevisiae. With initial sugar concentrations (S0) of 20 g/l, the yeasts were derepressed and the ethanol produced during the exponential phase was consumed in a diauxic phase. The rate of ethanol consumption decreased with increasing S0 and disappeared at 250 g/l when the Crabtree effect was complete and almost all the sugar consumed was transformed into ethanol with a yield factor of 0.42 g/g. Sucrose hydrolysis was delayed at high S0 because of glucose repression of invertase synthesis, which was triggered at concentrations above 40 g/l. At S0 higher than 250 g/l, even when glucose had been exhausted, sucrose was hydrolyzed very slowly, probably due to an inhibition at this low water activity. Although with lower metabolic rates and longer times of fermentation, 250 g/l is considered the optimal initial concentration because it avoids the diauxic consumption of ethanol and maintains enough invertase activity to consume all the sucrose, and also avoids the inhibitions due to lower water activities at higher S0.pt_PT
dc.description.sponsorshipProject Alfaetilico, as part of the Portuguese National Program QREN-POAlgarve21pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.4014/jmb.1408.08015pt_PT
dc.identifier.issn1738-8872
dc.identifier.urihttp://hdl.handle.net/10400.1/13920
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherKorean Society for Microbiology and Biotechnologypt_PT
dc.relation.publisherversionhttp://www.jmb.or.kr/journal/view.html?doi=10.4014/jmb.1408.08015pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectEthanolpt_PT
dc.subjectFermentationpt_PT
dc.subjectGalactanspt_PT
dc.subjectGene expression regulationpt_PT
dc.subjectIndustrial wastept_PT
dc.subjectMannanspt_PT
dc.subjectPlant gumspt_PT
dc.subjectSaccharomyces cerevisiaept_PT
dc.subjectbeta-Fructofuranosidasept_PT
dc.subjectBioethanolpt_PT
dc.subjectCarob podpt_PT
dc.subjectInvertase synthesispt_PT
dc.subjectSaccharomycespt_PT
dc.subjectSecond-generation biofuelspt_PT
dc.titleKinetic and energetic parameters of carob wastes fermentation by Saccharomyces cerevisiae: crabtree effect, ethanol toxicity, and invertase repressionpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage844pt_PT
oaire.citation.issue6pt_PT
oaire.citation.startPage837pt_PT
oaire.citation.titleJournal of microbiology and biotechnologypt_PT
oaire.citation.volume25pt_PT
person.familyNameRodrigues
person.familyNameRaposo
person.familyNameConstantino
person.familyNameQuintas
person.familyNameLima Costa
person.givenNameBrígida
person.givenNameSara
person.givenNameAna
person.givenNameCélia
person.givenNameMaria Emília
person.identifier.ciencia-id631C-A162-3F1E
person.identifier.ciencia-idF010-54F2-9E30
person.identifier.ciencia-idEA14-D76B-FF6E
person.identifier.orcid0000-0001-9495-0813
person.identifier.orcid0000-0001-5344-7349
person.identifier.orcid0000-0002-9968-3512
person.identifier.orcid0000-0001-9680-5089
person.identifier.orcid0000-0002-4433-5756
person.identifier.ridL-3673-2013
person.identifier.scopus-author-id7801639810
person.identifier.scopus-author-id7003633118
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication99ea34cc-7325-4ae7-b288-31d7e43ab28f
relation.isAuthorOfPublication035b3010-5f47-4700-b196-402970ff2a69
relation.isAuthorOfPublication466ef81c-c32f-4829-a96e-97d37c157fc0
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relation.isAuthorOfPublication6eb9b8a6-dd51-4ace-809b-7d7de9cdfe36
relation.isAuthorOfPublication.latestForDiscovery035b3010-5f47-4700-b196-402970ff2a69

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